Design, Synthesis, and Characterization of Small-Molecule Reagents That Cooperatively Provide Dual Readouts for Triaging and, When Necessary, Quantifying Point-of-Need Enzyme Assays

Adam D. Brooks, Hemakesh Mohapatra, Scott T. Phillips

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A newly designed small molecule reagent provides both qualitative and quantitative readouts in assays that detect enzyme biomarkers. The qualitative readout enables rapid triaging of samples so that only samples that contain relevant concentrations of the target analyte must be quantified. The reagent is accessible in essentially three steps and 34% overall yield, is stable as a solid when heated to 44 °C for >1 month, and does not produce background signal when used in an assay. This paper describes the design and synthesis of the reagent, characterizes its response properties, and establishes the scope of its reactivity.

Original languageEnglish (US)
Pages (from-to)10437-10445
Number of pages9
JournalJournal of Organic Chemistry
Volume80
Issue number21
DOIs
StatePublished - Nov 6 2015

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

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