Designer biofilms: Controlling biofilm formation and dispersal using a synthetic quorum sensing circuit in microfluidic devices

Manjunath Hegde, Jeongyun Kim, Seok Hoon Hong, Thomas Keith Wood, Arul Jayaraman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Biofilms are bacterial communities that are associated with infections and biofouling. Hence, it is important to control biofilm formation. We proposed to manipulate biofilm formation using tools of synthetic biology and protein engineering. Previously, we engineered two Escherichia coli proteins that promote dispersal of mono-species biofilms. The global regulator Hha was engineered to cause cell death [1] and BdcA was engineered to reduce concentrations of the second messenger 3,5-cyclic diguanylic acid (c-di-GMP) [2]. Here, we constructed a synthetic biofilm circuit that utilizes the Hha and BdcA dispersal proteins along with a quorum sensing (QS) switch that works based on the accumulation of the signal N-(3-oxo-dodecanoyl)-L-homoserine lactone (3-o-C12HSL) and implemented it in a microfluidic biofilm engineering (μBE) device.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1554-1556
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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