Detecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer

Fu Sheng Wang, R. M. Rowan, M. Creer, A. Hay, M. Dorfner, S. Peesapati, B. Connell, Y. Nakamura, A. Inagaki, I. Otani, Y. Hamaguchi, K. Hirai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In clinical medicine, particularly in the newly developing stem cell therapies required to support the practice of regenerative medicine, the measurement of both CD34+ and CD34- hematopoietic stem cells (HSC)/hematopoietic progenitor cells (HPC) is important in obtaining more accurate information about the total HSC/HPC content in various stem/progenitor cell sources. We report the results of an investigation into methods of detecting CD34+ and CD34- HSC/HPC using the immature information (IMI) channel incorporated into the Sysmex XE-2100 and SE-9000 automated hematology analyzers. In this study, CD34+ and CD34- HSC/HPC were separated by immunologic methods and quantified by flow cytometry (FACScan) and IMI channel analysis. In addition, CD34-/CD133+ HSC were prepared by a sequential antibody-based positive selection strategy. These cells appeared in the same area as CD34+ cells in the IMI channel of the automated hematology analyzer. These findings confirmed that an automated hematology analyzer can be used to measure both CD34+ and CD34- HSC. These results may explain the difference in HSC/HPC counts sometimes observed between the automated hematology analyzer and flow cytometric methods for CD34+ measurement. The results of this study demonstrated the potential of automated cell counting methods for measuring HSC content in cellular products for both research and clinical applications.

Original languageEnglish (US)
Pages (from-to)200-205
Number of pages6
JournalLaboratory Hematology
Volume10
Issue number4
DOIs
StatePublished - Dec 1 2004

Fingerprint

Hematology
Hematopoietic Stem Cells
Stem cells
Stem Cells
Flow cytometry
Regenerative Medicine
Medicine
Clinical Medicine
Cell- and Tissue-Based Therapy
Flow Cytometry
Cell Count
Antibodies

All Science Journal Classification (ASJC) codes

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Wang, Fu Sheng ; Rowan, R. M. ; Creer, M. ; Hay, A. ; Dorfner, M. ; Peesapati, S. ; Connell, B. ; Nakamura, Y. ; Inagaki, A. ; Otani, I. ; Hamaguchi, Y. ; Hirai, K. / Detecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer. In: Laboratory Hematology. 2004 ; Vol. 10, No. 4. pp. 200-205.
@article{b08fbcd583614f9b9ab1790e4a078517,
title = "Detecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer",
abstract = "In clinical medicine, particularly in the newly developing stem cell therapies required to support the practice of regenerative medicine, the measurement of both CD34+ and CD34- hematopoietic stem cells (HSC)/hematopoietic progenitor cells (HPC) is important in obtaining more accurate information about the total HSC/HPC content in various stem/progenitor cell sources. We report the results of an investigation into methods of detecting CD34+ and CD34- HSC/HPC using the immature information (IMI) channel incorporated into the Sysmex XE-2100 and SE-9000 automated hematology analyzers. In this study, CD34+ and CD34- HSC/HPC were separated by immunologic methods and quantified by flow cytometry (FACScan) and IMI channel analysis. In addition, CD34-/CD133+ HSC were prepared by a sequential antibody-based positive selection strategy. These cells appeared in the same area as CD34+ cells in the IMI channel of the automated hematology analyzer. These findings confirmed that an automated hematology analyzer can be used to measure both CD34+ and CD34- HSC. These results may explain the difference in HSC/HPC counts sometimes observed between the automated hematology analyzer and flow cytometric methods for CD34+ measurement. The results of this study demonstrated the potential of automated cell counting methods for measuring HSC content in cellular products for both research and clinical applications.",
author = "Wang, {Fu Sheng} and Rowan, {R. M.} and M. Creer and A. Hay and M. Dorfner and S. Peesapati and B. Connell and Y. Nakamura and A. Inagaki and I. Otani and Y. Hamaguchi and K. Hirai",
year = "2004",
month = "12",
day = "1",
doi = "10.1532/LH96.04057",
language = "English (US)",
volume = "10",
pages = "200--205",
journal = "Laboratory Hematology",
issn = "1080-2924",
publisher = "Carden Jennings Publishing Co. Ltd",
number = "4",

}

Wang, FS, Rowan, RM, Creer, M, Hay, A, Dorfner, M, Peesapati, S, Connell, B, Nakamura, Y, Inagaki, A, Otani, I, Hamaguchi, Y & Hirai, K 2004, 'Detecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer', Laboratory Hematology, vol. 10, no. 4, pp. 200-205. https://doi.org/10.1532/LH96.04057

Detecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer. / Wang, Fu Sheng; Rowan, R. M.; Creer, M.; Hay, A.; Dorfner, M.; Peesapati, S.; Connell, B.; Nakamura, Y.; Inagaki, A.; Otani, I.; Hamaguchi, Y.; Hirai, K.

In: Laboratory Hematology, Vol. 10, No. 4, 01.12.2004, p. 200-205.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer

AU - Wang, Fu Sheng

AU - Rowan, R. M.

AU - Creer, M.

AU - Hay, A.

AU - Dorfner, M.

AU - Peesapati, S.

AU - Connell, B.

AU - Nakamura, Y.

AU - Inagaki, A.

AU - Otani, I.

AU - Hamaguchi, Y.

AU - Hirai, K.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - In clinical medicine, particularly in the newly developing stem cell therapies required to support the practice of regenerative medicine, the measurement of both CD34+ and CD34- hematopoietic stem cells (HSC)/hematopoietic progenitor cells (HPC) is important in obtaining more accurate information about the total HSC/HPC content in various stem/progenitor cell sources. We report the results of an investigation into methods of detecting CD34+ and CD34- HSC/HPC using the immature information (IMI) channel incorporated into the Sysmex XE-2100 and SE-9000 automated hematology analyzers. In this study, CD34+ and CD34- HSC/HPC were separated by immunologic methods and quantified by flow cytometry (FACScan) and IMI channel analysis. In addition, CD34-/CD133+ HSC were prepared by a sequential antibody-based positive selection strategy. These cells appeared in the same area as CD34+ cells in the IMI channel of the automated hematology analyzer. These findings confirmed that an automated hematology analyzer can be used to measure both CD34+ and CD34- HSC. These results may explain the difference in HSC/HPC counts sometimes observed between the automated hematology analyzer and flow cytometric methods for CD34+ measurement. The results of this study demonstrated the potential of automated cell counting methods for measuring HSC content in cellular products for both research and clinical applications.

AB - In clinical medicine, particularly in the newly developing stem cell therapies required to support the practice of regenerative medicine, the measurement of both CD34+ and CD34- hematopoietic stem cells (HSC)/hematopoietic progenitor cells (HPC) is important in obtaining more accurate information about the total HSC/HPC content in various stem/progenitor cell sources. We report the results of an investigation into methods of detecting CD34+ and CD34- HSC/HPC using the immature information (IMI) channel incorporated into the Sysmex XE-2100 and SE-9000 automated hematology analyzers. In this study, CD34+ and CD34- HSC/HPC were separated by immunologic methods and quantified by flow cytometry (FACScan) and IMI channel analysis. In addition, CD34-/CD133+ HSC were prepared by a sequential antibody-based positive selection strategy. These cells appeared in the same area as CD34+ cells in the IMI channel of the automated hematology analyzer. These findings confirmed that an automated hematology analyzer can be used to measure both CD34+ and CD34- HSC. These results may explain the difference in HSC/HPC counts sometimes observed between the automated hematology analyzer and flow cytometric methods for CD34+ measurement. The results of this study demonstrated the potential of automated cell counting methods for measuring HSC content in cellular products for both research and clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=19944429023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944429023&partnerID=8YFLogxK

U2 - 10.1532/LH96.04057

DO - 10.1532/LH96.04057

M3 - Article

C2 - 15697089

AN - SCOPUS:19944429023

VL - 10

SP - 200

EP - 205

JO - Laboratory Hematology

JF - Laboratory Hematology

SN - 1080-2924

IS - 4

ER -