Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits

Chenghai Peng, Yuanshi Li, Huijuan Liang, Jiali Cheng, Qingsong Li, Xin Sun, Zhitao Li, Fengping Wang, Yuanyuan Guo, Zhen Tian, Liming Yang, Ye Tian, Zhiguo Zhang, Wenwu Cao

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Abstract

Photodynamic therapy (PDT) has been applied in the treatment of artery restenosis following balloon injury. This study aimed to detect the accumulation of 5-aminolevulinic acid (ALA)-derived protoporphyrin IX (PpIX) in inflamed atherosclerotic plaque in rabbit model and evaluate the efficacy of PDT. The inflamed atherosclerotic plaque in the common carotid artery was produced by combination of balloon denudation injury and high cholesterol diet. After intravenous administration of ALA, the fluorescence of PpIX in plaque was detected. At the peak time, the correlation between the fluorescence intensity of PpIX and the macrophage infiltration extent in plaque was analyzed. Subsequently, PDT (635 nm at 50 J/cm2) on the atherosclerotic plaques (n = 48) was performed and its effect was evaluated by histopathology and immunohistochemistry. The fluorescence intensity of PpIX in the plaque reached the peak 2 h after injection and was 12 times stronger than that of adjacent normal vessel segment, and has a positive correlation with the macrophage content (r = 0.794, P < 0.001). Compared with the control group, the plaque area was reduced by 59% (P < 0.001) at 4 week after PDT, the plaque macrophage content decreased by 56% at 1 week and 64% at 4 week respectively, the smooth muscle cells (SMCs) was depleted by 24% at 1 week (P < 0.05) and collagen content increased by 44% at 4 week (P < 0.05). It should be pointed out that the SMC content increased by 18% after PDT at 4 week compared with that at 1 week (P < 0.05). Our study demonstrated that the ALA-derived PpIX can be detected to reflect the macrophage content in the plaque. ALA mediated PDT could reduce macrophage content and inhibit plaque progression, indicating a promising approach to treat inflamed atherosclerotic plaques.

Original languageEnglish (US)
Pages (from-to)26-31
Number of pages6
JournalJournal of Photochemistry and Photobiology B: Biology
Volume102
Issue number1
DOIs
StatePublished - Jan 10 2011

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rabbits
Photochemotherapy
Atherosclerotic Plaques
arteries
macrophages
Carotid Arteries
therapy
Aminolevulinic Acid
Rabbits
Macrophages
smooth muscle
muscle cells
acids
Fluorescence
balloons
fluorescence
Smooth Muscle Myocytes
diets
Common Carotid Artery
cholesterol

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

Peng, Chenghai ; Li, Yuanshi ; Liang, Huijuan ; Cheng, Jiali ; Li, Qingsong ; Sun, Xin ; Li, Zhitao ; Wang, Fengping ; Guo, Yuanyuan ; Tian, Zhen ; Yang, Liming ; Tian, Ye ; Zhang, Zhiguo ; Cao, Wenwu. / Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits. In: Journal of Photochemistry and Photobiology B: Biology. 2011 ; Vol. 102, No. 1. pp. 26-31.
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title = "Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits",
abstract = "Photodynamic therapy (PDT) has been applied in the treatment of artery restenosis following balloon injury. This study aimed to detect the accumulation of 5-aminolevulinic acid (ALA)-derived protoporphyrin IX (PpIX) in inflamed atherosclerotic plaque in rabbit model and evaluate the efficacy of PDT. The inflamed atherosclerotic plaque in the common carotid artery was produced by combination of balloon denudation injury and high cholesterol diet. After intravenous administration of ALA, the fluorescence of PpIX in plaque was detected. At the peak time, the correlation between the fluorescence intensity of PpIX and the macrophage infiltration extent in plaque was analyzed. Subsequently, PDT (635 nm at 50 J/cm2) on the atherosclerotic plaques (n = 48) was performed and its effect was evaluated by histopathology and immunohistochemistry. The fluorescence intensity of PpIX in the plaque reached the peak 2 h after injection and was 12 times stronger than that of adjacent normal vessel segment, and has a positive correlation with the macrophage content (r = 0.794, P < 0.001). Compared with the control group, the plaque area was reduced by 59{\%} (P < 0.001) at 4 week after PDT, the plaque macrophage content decreased by 56{\%} at 1 week and 64{\%} at 4 week respectively, the smooth muscle cells (SMCs) was depleted by 24{\%} at 1 week (P < 0.05) and collagen content increased by 44{\%} at 4 week (P < 0.05). It should be pointed out that the SMC content increased by 18{\%} after PDT at 4 week compared with that at 1 week (P < 0.05). Our study demonstrated that the ALA-derived PpIX can be detected to reflect the macrophage content in the plaque. ALA mediated PDT could reduce macrophage content and inhibit plaque progression, indicating a promising approach to treat inflamed atherosclerotic plaques.",
author = "Chenghai Peng and Yuanshi Li and Huijuan Liang and Jiali Cheng and Qingsong Li and Xin Sun and Zhitao Li and Fengping Wang and Yuanyuan Guo and Zhen Tian and Liming Yang and Ye Tian and Zhiguo Zhang and Wenwu Cao",
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Peng, C, Li, Y, Liang, H, Cheng, J, Li, Q, Sun, X, Li, Z, Wang, F, Guo, Y, Tian, Z, Yang, L, Tian, Y, Zhang, Z & Cao, W 2011, 'Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits', Journal of Photochemistry and Photobiology B: Biology, vol. 102, no. 1, pp. 26-31. https://doi.org/10.1016/j.jphotobiol.2010.09.001

Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits. / Peng, Chenghai; Li, Yuanshi; Liang, Huijuan; Cheng, Jiali; Li, Qingsong; Sun, Xin; Li, Zhitao; Wang, Fengping; Guo, Yuanyuan; Tian, Zhen; Yang, Liming; Tian, Ye; Zhang, Zhiguo; Cao, Wenwu.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 102, No. 1, 10.01.2011, p. 26-31.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits

AU - Peng, Chenghai

AU - Li, Yuanshi

AU - Liang, Huijuan

AU - Cheng, Jiali

AU - Li, Qingsong

AU - Sun, Xin

AU - Li, Zhitao

AU - Wang, Fengping

AU - Guo, Yuanyuan

AU - Tian, Zhen

AU - Yang, Liming

AU - Tian, Ye

AU - Zhang, Zhiguo

AU - Cao, Wenwu

PY - 2011/1/10

Y1 - 2011/1/10

N2 - Photodynamic therapy (PDT) has been applied in the treatment of artery restenosis following balloon injury. This study aimed to detect the accumulation of 5-aminolevulinic acid (ALA)-derived protoporphyrin IX (PpIX) in inflamed atherosclerotic plaque in rabbit model and evaluate the efficacy of PDT. The inflamed atherosclerotic plaque in the common carotid artery was produced by combination of balloon denudation injury and high cholesterol diet. After intravenous administration of ALA, the fluorescence of PpIX in plaque was detected. At the peak time, the correlation between the fluorescence intensity of PpIX and the macrophage infiltration extent in plaque was analyzed. Subsequently, PDT (635 nm at 50 J/cm2) on the atherosclerotic plaques (n = 48) was performed and its effect was evaluated by histopathology and immunohistochemistry. The fluorescence intensity of PpIX in the plaque reached the peak 2 h after injection and was 12 times stronger than that of adjacent normal vessel segment, and has a positive correlation with the macrophage content (r = 0.794, P < 0.001). Compared with the control group, the plaque area was reduced by 59% (P < 0.001) at 4 week after PDT, the plaque macrophage content decreased by 56% at 1 week and 64% at 4 week respectively, the smooth muscle cells (SMCs) was depleted by 24% at 1 week (P < 0.05) and collagen content increased by 44% at 4 week (P < 0.05). It should be pointed out that the SMC content increased by 18% after PDT at 4 week compared with that at 1 week (P < 0.05). Our study demonstrated that the ALA-derived PpIX can be detected to reflect the macrophage content in the plaque. ALA mediated PDT could reduce macrophage content and inhibit plaque progression, indicating a promising approach to treat inflamed atherosclerotic plaques.

AB - Photodynamic therapy (PDT) has been applied in the treatment of artery restenosis following balloon injury. This study aimed to detect the accumulation of 5-aminolevulinic acid (ALA)-derived protoporphyrin IX (PpIX) in inflamed atherosclerotic plaque in rabbit model and evaluate the efficacy of PDT. The inflamed atherosclerotic plaque in the common carotid artery was produced by combination of balloon denudation injury and high cholesterol diet. After intravenous administration of ALA, the fluorescence of PpIX in plaque was detected. At the peak time, the correlation between the fluorescence intensity of PpIX and the macrophage infiltration extent in plaque was analyzed. Subsequently, PDT (635 nm at 50 J/cm2) on the atherosclerotic plaques (n = 48) was performed and its effect was evaluated by histopathology and immunohistochemistry. The fluorescence intensity of PpIX in the plaque reached the peak 2 h after injection and was 12 times stronger than that of adjacent normal vessel segment, and has a positive correlation with the macrophage content (r = 0.794, P < 0.001). Compared with the control group, the plaque area was reduced by 59% (P < 0.001) at 4 week after PDT, the plaque macrophage content decreased by 56% at 1 week and 64% at 4 week respectively, the smooth muscle cells (SMCs) was depleted by 24% at 1 week (P < 0.05) and collagen content increased by 44% at 4 week (P < 0.05). It should be pointed out that the SMC content increased by 18% after PDT at 4 week compared with that at 1 week (P < 0.05). Our study demonstrated that the ALA-derived PpIX can be detected to reflect the macrophage content in the plaque. ALA mediated PDT could reduce macrophage content and inhibit plaque progression, indicating a promising approach to treat inflamed atherosclerotic plaques.

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