Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

B. R. Gulati, S. Munir, D. P. Patnayak, S. M. Goyal, V. Kapur

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

Original languageEnglish (US)
Pages (from-to)2967-2970
Number of pages4
JournalJournal of clinical microbiology
Volume39
Issue number8
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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