Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

B. R. Gulati, S. Munir, D. P. Patnayak, S. M. Goyal, V. Kapur

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

Original languageEnglish (US)
Pages (from-to)2967-2970
Number of pages4
JournalJournal of clinical microbiology
Volume39
Issue number8
DOIs
StatePublished - Aug 23 2001

Fingerprint

Metapneumovirus
Nucleocapsid Proteins
Recombinant Proteins
Enzyme-Linked Immunosorbent Assay
Antibodies
Protein C
Proteins
Western Blotting
Escherichia coli
Serum

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

@article{2a59b958780b45f5a26990f6814c863c,
title = "Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay",
abstract = "The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.",
author = "Gulati, {B. R.} and S. Munir and Patnayak, {D. P.} and Goyal, {S. M.} and V. Kapur",
year = "2001",
month = "8",
day = "23",
doi = "10.1128/JCM.39.8.2967-2970.2001",
language = "English (US)",
volume = "39",
pages = "2967--2970",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "8",

}

Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay. / Gulati, B. R.; Munir, S.; Patnayak, D. P.; Goyal, S. M.; Kapur, V.

In: Journal of clinical microbiology, Vol. 39, No. 8, 23.08.2001, p. 2967-2970.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of antibodies to U.S. isolates of avian pneumovirus by a recombinant nucleocapsid protein-based sandwich enzyme-linked immunosorbent assay

AU - Gulati, B. R.

AU - Munir, S.

AU - Patnayak, D. P.

AU - Goyal, S. M.

AU - Kapur, V.

PY - 2001/8/23

Y1 - 2001/8/23

N2 - The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

AB - The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the ≈47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

UR - http://www.scopus.com/inward/record.url?scp=0034884541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034884541&partnerID=8YFLogxK

U2 - 10.1128/JCM.39.8.2967-2970.2001

DO - 10.1128/JCM.39.8.2967-2970.2001

M3 - Article

C2 - 11474024

AN - SCOPUS:0034884541

VL - 39

SP - 2967

EP - 2970

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 8

ER -