Detection of Escherichia coli Serogroups O26 and O113 by PCR Amplification of the wzx and wzy Genes

Chitrita DebRoy, Elisabeth Roberts, James Kundrat, Michael A. Davis, Connie E. Briggs, Pina M. Fratamico

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

PCR-based assays for detecting enterohemorrhagic Escherichia coli serogroups O26 and O113 were developed by targeting the wzx (O-antigen flippase) and the wzy (O-antigen polymerase) genes found in the O-antigen gene cluster of each organism. The PCR assays were specific for the respective serogroups, as there was no amplification of DNA from non-O26 and non-O113 E. coli serogroups or from other bacterial genera tested. Using the PCR assays, we were able to detect the organisms in seeded apple juice inoculated at concentration levels as low as ≤10 CFU/ml. The O26- and O113-specific PCR assays can potentially be used for typing E. coli O26 and O113 serogroups; these assays will offer an advantage to food and environmental microbiology laboratories in terms of identifying these non-O157 serogroups by replacing antigen-based serotyping.

Original languageEnglish (US)
Pages (from-to)1830-1832
Number of pages3
JournalApplied and environmental microbiology
Volume70
Issue number3
DOIs
StatePublished - Mar 1 2004

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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