Detection of Flavescence dorée phytoplasma in grapevine by reverse-transcription PCR

P. Margaria, C. Rosa, C. Marzachi, M. Turina, Sabrina Palmano

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Flavescence dorée (FD) is the most serious phytoplasma disease of grapevine. This report describes a novel method of detecting FD phytoplasma based on reverse-transcription polymerase chain reaction (RT-PCR) on 16S ribosomal RNA (16SrRNA) which will greatly improve mass screening of infected grapevines. A rapid protocol for extracting sap from whole leaves or midveins and successive one-tube amplification by RT-PCR was applied to grapevine samples with or without symptoms collected from different areas of Piedmont (northwestern Italy). Results were compared with those obtained using one of the current diagnostic methods that utilizes nested PCR on phytoplasma DNA-enriched preparations. A Cohen's kappa index of 0.76 indicated a substantial agreement between the two sets of results. The RT-PCR method has the advantage of being a rapid, reliable, and sensitive assay for large-scale screening of grapevines.

Original languageEnglish (US)
Pages (from-to)1496-1501
Number of pages6
JournalPlant disease
Volume91
Issue number11
DOIs
StatePublished - Nov 1 2007

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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