Detection of insertion/deletion polymorphisms from challenged samples using the Investigator DIPplex® Kit

Rebecca Klein, Cedric Neumann, Reena Roy

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

This research focuses on detection of bi-allelic insertion/deletion polymorphisms (InDels) from challenged samples using the Investigator DIPplex® Kit from Qiagen. The study included analyzing body fluids from humans, as well as pristine and degraded samples. For the purpose of assessing species specificity, samples from various animals were included. At first, an analytical threshold (AT) for the detection of alleles was established based on an assessment of the noise in the system. Then, InDel profiles were obtained from samples exposed to detrimental environmental conditions, washed bloodstains, lipsticks, ChapStick®, ancient Croatian bone samples, and every day products such as toothbrushes and dental floss. Concordant profiles were obtained from different body fluids of the same donor. InDel profiles were also generated successfully when body fluids were deposited on substrates and directly amplified without pre-treatment with buffer or washing reagents. InDels can provide additional information when only partial STR profiles are generated from challenged samples.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalForensic Science International: Genetics
Volume16
DOIs
Publication statusPublished - Jan 1 2015

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All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Genetics

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