Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies

Raghu Sinha, Sunil Arora, Usha Datta, Shobha Sehgal

Research output: Contribution to journalArticle

Abstract

Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was how-ever low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.

Original languageEnglish (US)
Pages (from-to)351-359
Number of pages9
JournalMicrobiology and Immunology
Volume36
Issue number4
StatePublished - Jan 1 1992

Fingerprint

Visceral Leishmaniasis
Leishmania
Monoclonal Antibodies
Antigens
Leishmania donovani
Immune Sera
Serum
Epitopes
Western Blotting
Enzyme-Linked Immunosorbent Assay
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Sinha, Raghu ; Arora, Sunil ; Datta, Usha ; Sehgal, Shobha. / Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies. In: Microbiology and Immunology. 1992 ; Vol. 36, No. 4. pp. 351-359.
@article{433f7ec38e744e2681828a72edb56d7d,
title = "Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies",
abstract = "Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30{\%} of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4{\%}. The efficacy of Hyb.6 was how-ever low (31.7{\%}). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.",
author = "Raghu Sinha and Sunil Arora and Usha Datta and Shobha Sehgal",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "36",
pages = "351--359",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "4",

}

Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies. / Sinha, Raghu; Arora, Sunil; Datta, Usha; Sehgal, Shobha.

In: Microbiology and Immunology, Vol. 36, No. 4, 01.01.1992, p. 351-359.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of Leishmania Antigen in Kala Azar Patients Using Monoclonal Antibodies

AU - Sinha, Raghu

AU - Arora, Sunil

AU - Datta, Usha

AU - Sehgal, Shobha

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was how-ever low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.

AB - Twenty-one monoclonal antibodies were produced against promastigote antigens of Leishmania donovani. Five monoclonal antibodies (Hyb.17, 6, 5, 4 and 2) identifying molecules associated with various L. donovani antigenic determinants ranging from 42–116 kDa were selected as ‘capture antibodies’ and compared with specific anti-leishmania antisera for detection of circulating leishmania antigens in kala azar patients’ sera in a competitive-enzyme-linked immunosorbent assay system (ELISA). The anti-leishmania antisera could detect circulating antigen in 30% of kala azar cases while out of the five monoclonals, Hyb.17 could effectively detect circulating leishmania antigen in 85.4%. The efficacy of Hyb.6 was how-ever low (31.7%). The antigens recognized by these monoclonal antibodies in the western blot assay could possibly represent the ones circulating in sera of patients suffering from kala azar. A cocktail of these monoclonal antibodies may be more useful than the conventional polyclonal antisera in detection of circulating antigen for clinical diagnosis of kala azar.

UR - http://www.scopus.com/inward/record.url?scp=85010152317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85010152317&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 351

EP - 359

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 4

ER -