Detection of LipL32-specific IgM by ELISA in sera of patients with a clinical diagnosis of leptospirosis

Kumaresan Vedhagiri, Sridhar Velineni, John F. Timoney, Santhanam Shanmughapriya, Paluru Vijayachari, Ramasamy Narayanan, Kalimuthusamy Natarajaseenivasan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Successful treatment of leptospirosis is heavily dependent on early diagnosis and prompt initiation of antibiotic therapy. An ELISA test to detect specific IgM antibodies against LipL32 for early diagnosis of leptospirosis is described and evaluated here. One thousand one hundred and eighty sera from clinically suspected leptospirosis cases were enrolled together with 109 healthy volunteers selected from an endemic area between October 2007 and January 2010. Patients were categorized based on their clinical signs and symptoms. Sera were screened for leptospiral antibodies by the microscopic agglutination test (MAT) using a panel of locally circulating serovars followed by enzyme-linked immunosorbent assay (ELISA) based on recombinant LipL32 from Leptospira interrogans serovar Autumnalis strain N2. The sensitivity and specificity of the ELISA test were determined to establish its diagnostic efficiency. The cutoff value was determined to be 0.205. Overall sensitivity and specificity compared to the MAT were found to be 96.4 and 90.4%, respectively. The LipL32-specific IgM ELISA had good sensitivity and acceptable specificity and may be a candidate for the early serodiagnosis of human leptospirosis.

Original languageEnglish (US)
Pages (from-to)130-135
Number of pages6
JournalPathogens and Global Health
Volume107
Issue number3
DOIs
StatePublished - Apr 1 2013

Fingerprint

Leptospirosis
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Agglutination Tests
Leptospira interrogans serovar autumnalis
Serum
Sensitivity and Specificity
Early Diagnosis
Antibodies
Serologic Tests
Signs and Symptoms
Healthy Volunteers
Anti-Bacterial Agents
Therapeutics

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Vedhagiri, Kumaresan ; Velineni, Sridhar ; Timoney, John F. ; Shanmughapriya, Santhanam ; Vijayachari, Paluru ; Narayanan, Ramasamy ; Natarajaseenivasan, Kalimuthusamy. / Detection of LipL32-specific IgM by ELISA in sera of patients with a clinical diagnosis of leptospirosis. In: Pathogens and Global Health. 2013 ; Vol. 107, No. 3. pp. 130-135.
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Vedhagiri, K, Velineni, S, Timoney, JF, Shanmughapriya, S, Vijayachari, P, Narayanan, R & Natarajaseenivasan, K 2013, 'Detection of LipL32-specific IgM by ELISA in sera of patients with a clinical diagnosis of leptospirosis', Pathogens and Global Health, vol. 107, no. 3, pp. 130-135. https://doi.org/10.1179/2047773213Y.0000000088

Detection of LipL32-specific IgM by ELISA in sera of patients with a clinical diagnosis of leptospirosis. / Vedhagiri, Kumaresan; Velineni, Sridhar; Timoney, John F.; Shanmughapriya, Santhanam; Vijayachari, Paluru; Narayanan, Ramasamy; Natarajaseenivasan, Kalimuthusamy.

In: Pathogens and Global Health, Vol. 107, No. 3, 01.04.2013, p. 130-135.

Research output: Contribution to journalArticle

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AU - Vedhagiri, Kumaresan

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AU - Shanmughapriya, Santhanam

AU - Vijayachari, Paluru

AU - Narayanan, Ramasamy

AU - Natarajaseenivasan, Kalimuthusamy

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N2 - Successful treatment of leptospirosis is heavily dependent on early diagnosis and prompt initiation of antibiotic therapy. An ELISA test to detect specific IgM antibodies against LipL32 for early diagnosis of leptospirosis is described and evaluated here. One thousand one hundred and eighty sera from clinically suspected leptospirosis cases were enrolled together with 109 healthy volunteers selected from an endemic area between October 2007 and January 2010. Patients were categorized based on their clinical signs and symptoms. Sera were screened for leptospiral antibodies by the microscopic agglutination test (MAT) using a panel of locally circulating serovars followed by enzyme-linked immunosorbent assay (ELISA) based on recombinant LipL32 from Leptospira interrogans serovar Autumnalis strain N2. The sensitivity and specificity of the ELISA test were determined to establish its diagnostic efficiency. The cutoff value was determined to be 0.205. Overall sensitivity and specificity compared to the MAT were found to be 96.4 and 90.4%, respectively. The LipL32-specific IgM ELISA had good sensitivity and acceptable specificity and may be a candidate for the early serodiagnosis of human leptospirosis.

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