Detection of Pyricularia grisea causing gray leaf spot of perennial ryegrass turf by a rapid immuno-recognition assay

Wakar Uddin, G. Viji, G. L. Schumann, S. H. Boyd

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An immuno-recognition assay using a monoclonal antibody was developed to detect Pyricularia grisea, the causal agent of gray leaf spot of perennial ryegrass (Lolium perenne). In vitro assays with isolates of P. grisea from perennial ryegrass, tall fescue (Festuca arundinacea), St. Augustinegrass (Stenotaphrum secundatum), crabgrass (Digitaria sanguinalis), finger millet (Eleusine coracana), wheat (Triticum aestivum), triticale (x Triticosecale rimpaui), and rice (Oryza sativa) showed positive reactions; however, the strength of the reactions differed among isolates. Reactions were more intense with isolates from perennial ryegrass, wheat, and triticale. All P. grisea isolates from perennial ryegrass collected from various regions of the United States showed positive reactions, P. grisea was detected at antigen dilution rates of 0.5x, 0.25x, 0.13x, 0.06x, and 0.03x. Dot-blot assays with Bipolaris sorokiniana, Colletotrichum graminicola, Curvularia lunata, Microdochium nivale, Pythium aphanidermatum, Rhizoctonia solani, or Sclerotinia homoeocarpa isolated from turfgrasses were negative. In vivo assays of symptomatic leaves of perennial ryegrass plants inoculated with P. grisea also showed positive reactions, and those inoculated with B. sorokiniana, P. aphanidermatum, R. solani, or S. homoeocarpa were negative. Intensity of reaction between the monoclonal antibody and P. grisea was proportional to disease severity in perennial ryegrass inoculated with P. grisea; however, there was no reaction in dot blots of leaf tissue collected during the latent period. P. grisea was detected in perennial ryegrass samples from golf course fairways affected by gray leaf spot in Connecticut, Massachusetts, Maine, New Jersey, Pennsylvania, and Rhode Island using this procedure. The monoclonal antibody recognition system is highly sensitive to P. grisea and can be used effectively for the rapid diagnosis of gray leaf spot of perennial ryegrass turf.

Original languageEnglish (US)
Pages (from-to)772-778
Number of pages7
JournalPlant disease
Volume87
Issue number7
DOIs
StatePublished - Jul 1 2003

Fingerprint

leaf spot
Magnaporthe grisea
lawns and turf
Lolium perenne
assays
Sclerotinia homoeocarpa
Pythium aphanidermatum
Triticosecale
Bipolaris sorokiniana
monoclonal antibodies
Festuca arundinacea
Thanatephorus cucumeris
Glomerella graminicola
Stenotaphrum secundatum
Monographella nivalis
Digitaria sanguinalis
Eleusine coracana
wheat
golf courses
latent period

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Detection of Pyricularia grisea causing gray leaf spot of perennial ryegrass turf by a rapid immuno-recognition assay",
abstract = "An immuno-recognition assay using a monoclonal antibody was developed to detect Pyricularia grisea, the causal agent of gray leaf spot of perennial ryegrass (Lolium perenne). In vitro assays with isolates of P. grisea from perennial ryegrass, tall fescue (Festuca arundinacea), St. Augustinegrass (Stenotaphrum secundatum), crabgrass (Digitaria sanguinalis), finger millet (Eleusine coracana), wheat (Triticum aestivum), triticale (x Triticosecale rimpaui), and rice (Oryza sativa) showed positive reactions; however, the strength of the reactions differed among isolates. Reactions were more intense with isolates from perennial ryegrass, wheat, and triticale. All P. grisea isolates from perennial ryegrass collected from various regions of the United States showed positive reactions, P. grisea was detected at antigen dilution rates of 0.5x, 0.25x, 0.13x, 0.06x, and 0.03x. Dot-blot assays with Bipolaris sorokiniana, Colletotrichum graminicola, Curvularia lunata, Microdochium nivale, Pythium aphanidermatum, Rhizoctonia solani, or Sclerotinia homoeocarpa isolated from turfgrasses were negative. In vivo assays of symptomatic leaves of perennial ryegrass plants inoculated with P. grisea also showed positive reactions, and those inoculated with B. sorokiniana, P. aphanidermatum, R. solani, or S. homoeocarpa were negative. Intensity of reaction between the monoclonal antibody and P. grisea was proportional to disease severity in perennial ryegrass inoculated with P. grisea; however, there was no reaction in dot blots of leaf tissue collected during the latent period. P. grisea was detected in perennial ryegrass samples from golf course fairways affected by gray leaf spot in Connecticut, Massachusetts, Maine, New Jersey, Pennsylvania, and Rhode Island using this procedure. The monoclonal antibody recognition system is highly sensitive to P. grisea and can be used effectively for the rapid diagnosis of gray leaf spot of perennial ryegrass turf.",
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Detection of Pyricularia grisea causing gray leaf spot of perennial ryegrass turf by a rapid immuno-recognition assay. / Uddin, Wakar; Viji, G.; Schumann, G. L.; Boyd, S. H.

In: Plant disease, Vol. 87, No. 7, 01.07.2003, p. 772-778.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of Pyricularia grisea causing gray leaf spot of perennial ryegrass turf by a rapid immuno-recognition assay

AU - Uddin, Wakar

AU - Viji, G.

AU - Schumann, G. L.

AU - Boyd, S. H.

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N2 - An immuno-recognition assay using a monoclonal antibody was developed to detect Pyricularia grisea, the causal agent of gray leaf spot of perennial ryegrass (Lolium perenne). In vitro assays with isolates of P. grisea from perennial ryegrass, tall fescue (Festuca arundinacea), St. Augustinegrass (Stenotaphrum secundatum), crabgrass (Digitaria sanguinalis), finger millet (Eleusine coracana), wheat (Triticum aestivum), triticale (x Triticosecale rimpaui), and rice (Oryza sativa) showed positive reactions; however, the strength of the reactions differed among isolates. Reactions were more intense with isolates from perennial ryegrass, wheat, and triticale. All P. grisea isolates from perennial ryegrass collected from various regions of the United States showed positive reactions, P. grisea was detected at antigen dilution rates of 0.5x, 0.25x, 0.13x, 0.06x, and 0.03x. Dot-blot assays with Bipolaris sorokiniana, Colletotrichum graminicola, Curvularia lunata, Microdochium nivale, Pythium aphanidermatum, Rhizoctonia solani, or Sclerotinia homoeocarpa isolated from turfgrasses were negative. In vivo assays of symptomatic leaves of perennial ryegrass plants inoculated with P. grisea also showed positive reactions, and those inoculated with B. sorokiniana, P. aphanidermatum, R. solani, or S. homoeocarpa were negative. Intensity of reaction between the monoclonal antibody and P. grisea was proportional to disease severity in perennial ryegrass inoculated with P. grisea; however, there was no reaction in dot blots of leaf tissue collected during the latent period. P. grisea was detected in perennial ryegrass samples from golf course fairways affected by gray leaf spot in Connecticut, Massachusetts, Maine, New Jersey, Pennsylvania, and Rhode Island using this procedure. The monoclonal antibody recognition system is highly sensitive to P. grisea and can be used effectively for the rapid diagnosis of gray leaf spot of perennial ryegrass turf.

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