Detection of senescence-associated heterochromatin foci (SAHF)

Katherine M. Aird, Rugang Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

48 Scopus citations

Abstract

One of the most prominent features of cellular senescence, a stress response that prevents the propagation of cells that have accumulated potentially oncogenic alterations, is a permanent loss of proliferative potential. Thus, at odds with quiescent cells, which resume proliferation when stimulated to do so, senescent cells cannot proceed through the cell cycle even in the presence of mitogenic factors. Here, we describe a set of cyto fluorometric techniques for studying how chemical and/or physical stimuli alter the cell cycle in vitro, in both qualitative and quantitative terms. Taken together, these methods allow for the identification of bonafide cytostatic effects as well as for a refined characterization of cell cycle distributions, providing information on proliferation, DNA content, as well as the presence of cell cycle phase-specific markers. At the end of the chapter, a set of guidelines is offered to assist researchers that approach the study of the cell cycle with the interpretation of results.

Original languageEnglish (US)
Title of host publicationCell Senescence
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages185-196
Number of pages12
ISBN (Print)9781627032384
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume965
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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