Detection of sepsis in patient blood samples using CD64 expression in a microfluidic cell separation device

Ye Zhang, Wenjie Li, Yun Zhou, Amanda Johnson, Amanda Venable, Ahmed Hassan, John Griswold, Dimitri Pappas

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A microfluidic affinity separation device was developed for the detection of sepsis in critical care patients. An affinity capture method was developed to capture cells based on changes in CD64 expression in a single, simple microfluidic chip for sepsis detection. Both sepsis patient samples and a laboratory CD64+ expression model were used to validate the microfluidic assay. Flow cytometry analysis showed that the chip cell capture had a linear relationship with CD64 expression in laboratory models. The Sepsis Chip detected an increase in upregulated neutrophil-like cells when the upregulated cell population is as low as 10% of total cells spiked into commercially available aseptic blood samples. In a proof of concept study, blood samples obtained from sepsis patients within 24 hours of diagnosis were tested on the chip to further validate its performance. On-chip CD64+ cell capture from 10 patient samples (619 ± 340 cells per chip) was significantly different from control samples (32 ± 11 cells per chip) and healthy volunteer samples (228 ± 95 cells per chip). In addition, the on-chip cell capture has a linear relationship with CD64 expression indicating our approach can be used to measure CD64 expression based on total cell capture on Sepsis Chip. Our method has proven to be sensitive, accurate, rapid, and cost-effective. Therefore, this device is a promising detection platform for neutrophil activation and sepsis diagnosis.

Original languageEnglish (US)
Pages (from-to)241-249
Number of pages9
JournalAnalyst
Volume143
Issue number1
DOIs
StatePublished - Jan 7 2018

Fingerprint

Microfluidics
Cell Separation
Sepsis
Blood
blood
Equipment and Supplies
capture method
Flow cytometry
flow cytometry
Assays
Chemical activation
Cells
assay
cost
detection
Costs
Neutrophil Activation
laboratory
Critical Care
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Zhang, Y., Li, W., Zhou, Y., Johnson, A., Venable, A., Hassan, A., ... Pappas, D. (2018). Detection of sepsis in patient blood samples using CD64 expression in a microfluidic cell separation device. Analyst, 143(1), 241-249. https://doi.org/10.1039/c7an01471f
Zhang, Ye ; Li, Wenjie ; Zhou, Yun ; Johnson, Amanda ; Venable, Amanda ; Hassan, Ahmed ; Griswold, John ; Pappas, Dimitri. / Detection of sepsis in patient blood samples using CD64 expression in a microfluidic cell separation device. In: Analyst. 2018 ; Vol. 143, No. 1. pp. 241-249.
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abstract = "A microfluidic affinity separation device was developed for the detection of sepsis in critical care patients. An affinity capture method was developed to capture cells based on changes in CD64 expression in a single, simple microfluidic chip for sepsis detection. Both sepsis patient samples and a laboratory CD64+ expression model were used to validate the microfluidic assay. Flow cytometry analysis showed that the chip cell capture had a linear relationship with CD64 expression in laboratory models. The Sepsis Chip detected an increase in upregulated neutrophil-like cells when the upregulated cell population is as low as 10{\%} of total cells spiked into commercially available aseptic blood samples. In a proof of concept study, blood samples obtained from sepsis patients within 24 hours of diagnosis were tested on the chip to further validate its performance. On-chip CD64+ cell capture from 10 patient samples (619 ± 340 cells per chip) was significantly different from control samples (32 ± 11 cells per chip) and healthy volunteer samples (228 ± 95 cells per chip). In addition, the on-chip cell capture has a linear relationship with CD64 expression indicating our approach can be used to measure CD64 expression based on total cell capture on Sepsis Chip. Our method has proven to be sensitive, accurate, rapid, and cost-effective. Therefore, this device is a promising detection platform for neutrophil activation and sepsis diagnosis.",
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Zhang, Y, Li, W, Zhou, Y, Johnson, A, Venable, A, Hassan, A, Griswold, J & Pappas, D 2018, 'Detection of sepsis in patient blood samples using CD64 expression in a microfluidic cell separation device', Analyst, vol. 143, no. 1, pp. 241-249. https://doi.org/10.1039/c7an01471f

Detection of sepsis in patient blood samples using CD64 expression in a microfluidic cell separation device. / Zhang, Ye; Li, Wenjie; Zhou, Yun; Johnson, Amanda; Venable, Amanda; Hassan, Ahmed; Griswold, John; Pappas, Dimitri.

In: Analyst, Vol. 143, No. 1, 07.01.2018, p. 241-249.

Research output: Contribution to journalArticle

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