Detection of shiga toxin-producing escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster

Chitrita Debroy, Elisabeth Roberts, Angela M. Valadez, Edward G. Dudley, Catherine N. Cutter

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

O-antigens on the surface of Escherichia coli are important virulence factors that are targets of both the innate and adaptive immune system and play a major role in pathogenicity. O-antigens that are responsible for antigenic specificity of the strain determine the O-serogroup. E. coli O26, O45, O103, O111, O113, O121, O145, and O157 have been the most commonly identified O-serogroups associated with Shiga toxin-producing E. coli (STEC) implicated in outbreaks of human illness all over the world. A multiplex polymerase chain reaction assay was developed to simultaneously detect the eight STEC O-serogroups targeting the wzx (O-antigen-flippase) genes of all O-antigen gene clusters. The sensitivity of the multiplex polymerase chain reaction was found to be 10 colony forming units for each O-group when enriched in broth and 100 colony forming units when enriched in artificially inoculated apple juice diluted with tryptic soy broth for 16 h at 37°C. The method can be used for detecting STEC O-groups simultaneously and may be exploited for improving the safety of food products.

Original languageEnglish (US)
Pages (from-to)651-652
Number of pages2
JournalFoodborne pathogens and disease
Volume8
Issue number5
DOIs
StatePublished - May 1 2011

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

Fingerprint Dive into the research topics of 'Detection of shiga toxin-producing escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster'. Together they form a unique fingerprint.

Cite this