Determination of hematocrit from mean transit time of red blood cells and plasma in cremaster muscle of the rat

L. Cram, H. H. Lipowsky

Research output: Contribution to conferencePaper

Abstract

Indicator dilution methods were used to measure the mean transit time of bolus injections of fluorescently labeled red cells (MTTrbc) and plasma (MTTplasma) containing 5 gms% fluorescent albumin. Following injection of a bolus into the femoral artery contralateral to the cremaster muscle, intensity time curves of the bolus dispersion were obtained within functionally paired arterioles and venules of successive microvascular divisions. The intensity time curves were obtained by digitizing videodensitometric measurements recorded under fluorescence microscopy. The average microvascular hematocrit (Hm) present in the network between these two sites is evaluated assuming that the resident volume of either red cells or plasma is equal to the product of their volumetric flow and respective mean transit times, and the actual discharge hematocrit (Hd) is assumed to be equal to the systemic hematocrit. The results demonstrate a general trend of diminishing Hm/Hd as blood flows from feeding to terminal arterioles, which agrees qualitatively with estimates obtained previously by direct cell counting and spectrophotometric methods.

Original languageEnglish (US)
Pages147-148
Number of pages2
StatePublished - Jan 1 1990
EventProceedings of the Sixteenth Annual Northeast Bioengineering Conference - University Park, PA, USA
Duration: Mar 26 1990Mar 27 1990

Other

OtherProceedings of the Sixteenth Annual Northeast Bioengineering Conference
CityUniversity Park, PA, USA
Period3/26/903/27/90

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All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Cram, L., & Lipowsky, H. H. (1990). Determination of hematocrit from mean transit time of red blood cells and plasma in cremaster muscle of the rat. 147-148. Paper presented at Proceedings of the Sixteenth Annual Northeast Bioengineering Conference, University Park, PA, USA, .