Determination of muscle-specific glucose flux using radioactive stereoisomers and microdialysis

David A. Maclean, Steven Ettinger, Lawrence Sinoway, Kathryn F. Lanoue

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The purpose of the present study was to evaluate a novel approach for determining skeletal muscle-specific glucose flux using radioactive stereoisomers and the microdialysis technique. Microdialysis probes were inserted into the vastus lateralis muscle of human subjects and perfused (4 μl/min) with a Ringer solution containing small amounts of radioactive D- and L-glucose as the internal reference markers for determining probe recovery as well as varying concentrations of insulin (0-10 μM). The rationale behind this approach was that both stereoisomers would be equally affected by the factors that determine probe recovery, with the exception of L-glucose, which is nonmetabolizable and would not be influenced by tissue uptake. Therefore, any differences in the probe recovery ratios between the D- and L-stereoisomers represent changes in skeletal muscle glucose uptake directly at the tissue level. There were no differences in probe recovery between the D- (42.3 ± 3.5%) and L- (41.2 ± 3.5) stereoisomers during the control period (no insulin), which resulted in a D/L ratio of 1.04 ± 0.03. However, during insulin perfusion (1 μM), The D/L ratio increased to 1.62 ± 0.08 and 1.58 ± 0.07 (P < 0.05) during the two collection (0-15 and 15-30 min) periods, respectively. This was accomplished solely by an increase (P < 0.05) in D-glucose probe recovery, as L-glucose probe recovery remained unchanged. In a second set of experiments, the perfusion of 10 μM insulin did not increase the D/L ratio (1.40 ± 0.11) above that observed during 1.0 μM (1.41 ± 0.07) insulin perfusion. These data suggest that this method is sufficiently sensitive to detect differences in insulin-stimulated glucose uptake; thus the use of radioactive stereoisomers in conjunction with the microdialysis technique provides a novel and useful technique for determining tissue-specific glucose flux and insulin sensitivity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume280
Issue number1 43-1
StatePublished - Jun 11 2001

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Stereoisomerism
Microdialysis
Glucose
Muscles
Insulin
Perfusion
Skeletal Muscle
Quadriceps Muscle
Insulin Resistance

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

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title = "Determination of muscle-specific glucose flux using radioactive stereoisomers and microdialysis",
abstract = "The purpose of the present study was to evaluate a novel approach for determining skeletal muscle-specific glucose flux using radioactive stereoisomers and the microdialysis technique. Microdialysis probes were inserted into the vastus lateralis muscle of human subjects and perfused (4 μl/min) with a Ringer solution containing small amounts of radioactive D- and L-glucose as the internal reference markers for determining probe recovery as well as varying concentrations of insulin (0-10 μM). The rationale behind this approach was that both stereoisomers would be equally affected by the factors that determine probe recovery, with the exception of L-glucose, which is nonmetabolizable and would not be influenced by tissue uptake. Therefore, any differences in the probe recovery ratios between the D- and L-stereoisomers represent changes in skeletal muscle glucose uptake directly at the tissue level. There were no differences in probe recovery between the D- (42.3 ± 3.5{\%}) and L- (41.2 ± 3.5) stereoisomers during the control period (no insulin), which resulted in a D/L ratio of 1.04 ± 0.03. However, during insulin perfusion (1 μM), The D/L ratio increased to 1.62 ± 0.08 and 1.58 ± 0.07 (P < 0.05) during the two collection (0-15 and 15-30 min) periods, respectively. This was accomplished solely by an increase (P < 0.05) in D-glucose probe recovery, as L-glucose probe recovery remained unchanged. In a second set of experiments, the perfusion of 10 μM insulin did not increase the D/L ratio (1.40 ± 0.11) above that observed during 1.0 μM (1.41 ± 0.07) insulin perfusion. These data suggest that this method is sufficiently sensitive to detect differences in insulin-stimulated glucose uptake; thus the use of radioactive stereoisomers in conjunction with the microdialysis technique provides a novel and useful technique for determining tissue-specific glucose flux and insulin sensitivity.",
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Determination of muscle-specific glucose flux using radioactive stereoisomers and microdialysis. / Maclean, David A.; Ettinger, Steven; Sinoway, Lawrence; Lanoue, Kathryn F.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 280, No. 1 43-1, 11.06.2001.

Research output: Contribution to journalArticle

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