Determination of polycyclic aromatic hydrocarbons in urine by solid phase extraction and GC/HRMS

Wenlin Huang, Christopher J. Smith, Charisse J. Walcott, Zheng Li, Selvin Edwards, Melvin Bullock, James Grainger, Donald George Jr Patterson

Research output: Contribution to conferencePaper

Abstract

The polycyclic aromatic hydrocarbons (PAH) in urine were analyzed using analytic method of solid phase extraction (SPE). The urine samples were spiked with 9 carbon-13 labeled standards which were hydrolyzed by β- glucuronidase/arylsulfatase. The solutions were blown in an evaporator and extracts were reconstituted in toluene. The method detection limits (MDL) ranged from 2 ng/L for 1-hydroxypyrene to 43.5 ng/L for 1-hydroxynaphthalene which produced good specificity, precision, linearity and recovery.

Original languageEnglish (US)
Pages683-684
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Arylsulfatases
Glucuronidase
Polycyclic Aromatic Hydrocarbons
Toluene
Evaporators
Carbon
Recovery
1-naphthol
1-hydroxypyrene

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Huang, W., Smith, C. J., Walcott, C. J., Li, Z., Edwards, S., Bullock, M., ... Patterson, D. G. J. (2002). Determination of polycyclic aromatic hydrocarbons in urine by solid phase extraction and GC/HRMS. 683-684. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.
Huang, Wenlin ; Smith, Christopher J. ; Walcott, Charisse J. ; Li, Zheng ; Edwards, Selvin ; Bullock, Melvin ; Grainger, James ; Patterson, Donald George Jr. / Determination of polycyclic aromatic hydrocarbons in urine by solid phase extraction and GC/HRMS. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.
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abstract = "The polycyclic aromatic hydrocarbons (PAH) in urine were analyzed using analytic method of solid phase extraction (SPE). The urine samples were spiked with 9 carbon-13 labeled standards which were hydrolyzed by β- glucuronidase/arylsulfatase. The solutions were blown in an evaporator and extracts were reconstituted in toluene. The method detection limits (MDL) ranged from 2 ng/L for 1-hydroxypyrene to 43.5 ng/L for 1-hydroxynaphthalene which produced good specificity, precision, linearity and recovery.",
author = "Wenlin Huang and Smith, {Christopher J.} and Walcott, {Charisse J.} and Zheng Li and Selvin Edwards and Melvin Bullock and James Grainger and Patterson, {Donald George Jr}",
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Huang, W, Smith, CJ, Walcott, CJ, Li, Z, Edwards, S, Bullock, M, Grainger, J & Patterson, DGJ 2002, 'Determination of polycyclic aromatic hydrocarbons in urine by solid phase extraction and GC/HRMS' Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02, pp. 683-684.

Determination of polycyclic aromatic hydrocarbons in urine by solid phase extraction and GC/HRMS. / Huang, Wenlin; Smith, Christopher J.; Walcott, Charisse J.; Li, Zheng; Edwards, Selvin; Bullock, Melvin; Grainger, James; Patterson, Donald George Jr.

2002. 683-684 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conferencePaper

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AU - Huang, Wenlin

AU - Smith, Christopher J.

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AU - Bullock, Melvin

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Huang W, Smith CJ, Walcott CJ, Li Z, Edwards S, Bullock M et al. Determination of polycyclic aromatic hydrocarbons in urine by solid phase extraction and GC/HRMS. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.