Development and characterization of biodegradable nanocomposite injectables for orthopaedic applications based on polyphosphazenes

Swaminathan Sethuraman, Lakshmi S. Nair, Saadiq El-Amin, My Tien Nguyen, Anurima Singh, Yaser E. Greish, Harry R. Allcock, Paul W. Brown, Cato T. Laurencin

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Self-setting hydroxyapatite-biodegradable injectable composites are excellent candidates for applications in orthopaedics. We have previously demonstrated the feasibility of development of self-setting calcium-deficient nanocrystalline hydroxyapatite-polymer composites using different calcium phosphate precursors and biodegradable polyphosphazenes. This study aimed to evaluate these novel injectable composites as suitable materials for orthopaedic applications through evaluating their biomechanical properties, osteoblast cellular attachment and gene expression over time. Our studies demonstrated that the morphology of the composite groups (PNEA-CDHA, PNEA-CDSHA, PNEA 50mPh50-CDHA, PNEA50mPh50-CDSHA, PNEA50PhPh50-CDHA, and PNEA50PhPh 50-CDSHA) formed was similar and found to have micro- and nanoporous structures resembling trabecular bone. The osteoblast phenotypic marker of bone, alkaline phosphatase, was expressed by the cells on the surface of the composites throughout the study and was comparable to tissue-culture polystyrene (control). Furthermore, the cells seeded on the composites expressed the characteristic osteoblastic genes, such as type-I collagen, alkaline phosphatase, osteocalcin, osteopontin and bone sialoprotein, indicating osteoblast differentiation, maturation and mineralization. Within our injectable composite groups, significant gene expression levels were displayed (P < 0.05). These novel injectable biodegradable polyphosphazenes-calcium-deficient hydroxyapatites materials are promising candidates for orthopaedic applications.

Original languageEnglish (US)
Pages (from-to)733-752
Number of pages20
JournalJournal of Biomaterials Science, Polymer Edition
Volume22
Issue number4-6
DOIs
StatePublished - Jan 1 2011

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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