Development of a novel pretargeting system with bifunctional nucleic acid molecules

Jing Zhou, Boonchoy Soontornworajit, Matthew P. Snipes, Yong Wang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.

Original languageEnglish (US)
Pages (from-to)521-525
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume386
Issue number3
DOIs
StatePublished - Aug 28 2009

Fingerprint

Nucleic Acids
Molecules
Oligonucleotides
Labeling

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Zhou, Jing ; Soontornworajit, Boonchoy ; Snipes, Matthew P. ; Wang, Yong. / Development of a novel pretargeting system with bifunctional nucleic acid molecules. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 386, No. 3. pp. 521-525.
@article{f05741ab51724c18a43f0a78fa5ed461,
title = "Development of a novel pretargeting system with bifunctional nucleic acid molecules",
abstract = "This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.",
author = "Jing Zhou and Boonchoy Soontornworajit and Snipes, {Matthew P.} and Yong Wang",
year = "2009",
month = "8",
day = "28",
doi = "10.1016/j.bbrc.2009.06.090",
language = "English (US)",
volume = "386",
pages = "521--525",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

Development of a novel pretargeting system with bifunctional nucleic acid molecules. / Zhou, Jing; Soontornworajit, Boonchoy; Snipes, Matthew P.; Wang, Yong.

In: Biochemical and Biophysical Research Communications, Vol. 386, No. 3, 28.08.2009, p. 521-525.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development of a novel pretargeting system with bifunctional nucleic acid molecules

AU - Zhou, Jing

AU - Soontornworajit, Boonchoy

AU - Snipes, Matthew P.

AU - Wang, Yong

PY - 2009/8/28

Y1 - 2009/8/28

N2 - This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.

AB - This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.

UR - http://www.scopus.com/inward/record.url?scp=67650034249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650034249&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.06.090

DO - 10.1016/j.bbrc.2009.06.090

M3 - Article

C2 - 19545539

AN - SCOPUS:67650034249

VL - 386

SP - 521

EP - 525

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -