Abstract
This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 521-525 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 386 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 28 2009 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
}
Development of a novel pretargeting system with bifunctional nucleic acid molecules. / Zhou, Jing; Soontornworajit, Boonchoy; Snipes, Matthew P.; Wang, Yong.
In: Biochemical and Biophysical Research Communications, Vol. 386, No. 3, 28.08.2009, p. 521-525.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development of a novel pretargeting system with bifunctional nucleic acid molecules
AU - Zhou, Jing
AU - Soontornworajit, Boonchoy
AU - Snipes, Matthew P.
AU - Wang, Yong
PY - 2009/8/28
Y1 - 2009/8/28
N2 - This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.
AB - This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications.
UR - http://www.scopus.com/inward/record.url?scp=67650034249&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650034249&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.06.090
DO - 10.1016/j.bbrc.2009.06.090
M3 - Article
C2 - 19545539
AN - SCOPUS:67650034249
VL - 386
SP - 521
EP - 525
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -