Development of an improved protocol for the isolation and detection of enterobacter sakazakii (Cronobacter) from powdered infant formula

Yi Chen, Kwang Yong Song, Eric Wayne Brown, Keith A. Lampel

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F ehromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.

Original languageEnglish (US)
Pages (from-to)1016-1022
Number of pages7
JournalJournal of Food Protection
Volume73
Issue number6
DOIs
StatePublished - Jan 1 2010

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

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