Development of an Organic Lateral Resolution Test Device for Imaging Mass Spectrometry

Melissa K. Passarelli, Jun Wang, Amir Saeid Mohammadi, Raphael Trouillon, Ian Gilmore, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread function (ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer function (MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.

Original languageEnglish (US)
Pages (from-to)9473-9480
Number of pages8
JournalAnalytical Chemistry
Volume86
Issue number19
DOIs
StatePublished - Oct 7 2014

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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