Development of branchiomotor neurons in zebrafish

Anand Chandrasekhar, Cecilia B. Moens, James Warren, Jr., Charles B. Kimmel, John Y. Kuwada

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The mechanisms underlying neuronal specification and axonogenesis in the vertebrate hindbrain are poorly understood. To address these questions, we have employed anatomical methods and mutational analysis to characterize the branchiomotor neurons in the zebrafish embryo. The zebrafish branchiomotor system is similar to those in the chick and mouse, except for the location of the nVII and nIX branchiomotor neurons. Developmental analyses of genes expressed by branchiomotor neurons suggest that the different location of the nVII neurons in the zebrafish may result from cell migration. To gain insight into the mechanisms underlying the organization and axonogenesis of these neurons, we examined the development of the branchiomotor pathways in neuronal mutants. The valentino b337 mutation blocks the formation of rhombomeres 5 and 6, and severely affects the development of the nVII and nIX motor nuclei. The cyclops b16 mutation deletes ventral midline cells in the neural tube, and leads to a severe disruption of most branchiomotor nuclei and axon pathways. These results demonstrate that rhombomere-specific cues and ventral midline cells play important roles in the development of the branchiomotor pathways.

Original languageEnglish (US)
Pages (from-to)2633-2644
Number of pages12
JournalDevelopment
Volume124
Issue number13
StatePublished - Jul 1 1997

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Zebrafish
Neurons
Developmental Genes
Rhombencephalon
Mutation
Minocycline
Neural Tube
Cell Movement
Cues
Axons
Vertebrates
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Chandrasekhar, A., Moens, C. B., Warren, Jr., J., Kimmel, C. B., & Kuwada, J. Y. (1997). Development of branchiomotor neurons in zebrafish. Development, 124(13), 2633-2644.
Chandrasekhar, Anand ; Moens, Cecilia B. ; Warren, Jr., James ; Kimmel, Charles B. ; Kuwada, John Y. / Development of branchiomotor neurons in zebrafish. In: Development. 1997 ; Vol. 124, No. 13. pp. 2633-2644.
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Chandrasekhar, A, Moens, CB, Warren, Jr., J, Kimmel, CB & Kuwada, JY 1997, 'Development of branchiomotor neurons in zebrafish', Development, vol. 124, no. 13, pp. 2633-2644.

Development of branchiomotor neurons in zebrafish. / Chandrasekhar, Anand; Moens, Cecilia B.; Warren, Jr., James; Kimmel, Charles B.; Kuwada, John Y.

In: Development, Vol. 124, No. 13, 01.07.1997, p. 2633-2644.

Research output: Contribution to journalArticle

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Chandrasekhar A, Moens CB, Warren, Jr. J, Kimmel CB, Kuwada JY. Development of branchiomotor neurons in zebrafish. Development. 1997 Jul 1;124(13):2633-2644.