Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using illumina sequencing

Yawen Wu, Ruhua Zhang, Margaret Staton, Scott E. Schlarbaum, Mark V. Coggeshall, Jeanne Romero-Severson, John E. Carlson, Nicole Zembower, Haiying Liang, Yi Xu, Daniela I. Drautz-Moses, Stephan C. Schuster, Oliver Gailing

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.

Original languageEnglish (US)
Pages (from-to)343-350
Number of pages8
JournalAnnals of Forest Research
Volume60
Issue number2
DOIs
StatePublished - 2017

All Science Journal Classification (ASJC) codes

  • Forestry
  • Ecology
  • Plant Science

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    Wu, Y., Zhang, R., Staton, M., Schlarbaum, S. E., Coggeshall, M. V., Romero-Severson, J., Carlson, J. E., Zembower, N., Liang, H., Xu, Y., Drautz-Moses, D. I., Schuster, S. C., & Gailing, O. (2017). Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using illumina sequencing. Annals of Forest Research, 60(2), 343-350. https://doi.org/10.15287/afr.2017.819