Development of injectable citrate-based bioadhesive bone implants

Denghui Xie, Jinshan Guo, M. Reza Mehdizadeh, Richard T. Tran, Ruisong Chen, Dawei Sun, Guoying Qian, Dadi Jin, Xiaochun Bai, Jian Yang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Injectable bone implants have been widely used in bone tissue repairs, including the treatment of comminuted bone fractures (CBF). However, most injectable bone implants are not suitable for the treatment of CBF because of their weak tissue adhesion strengths and minimal osteoinduction. Citrate has been recently reported to promote bone formation through enhanced bioceramic integration and osteoinductivity. Herein, a novel injectable citrate-based mussel-inspired bioadhesive hydroxyapatite (iCMBA/HA) bone substitute was developed for CBF treatment. Note that iCMBA/HA can be set within 2-4 minutes and the as-prepared (wet) iCMBA/HA possesses low swelling ratios, compressive mechanical strengths of up to 3.2 ± 0.27 MPa, complete degradation in 30 days, suitable biocompatibility, and osteoinductivity. This is also the first time that citrate supplementation in osteogenic medium and citrate released from iCMBA/HA degradation has been demonstrated to promote the mineralization of osteoblastic differentiated human mesenchymal stem cells (hMSCs). In vivo evaluation of iCMBA/HA in a rabbit comminuted radial fracture model showed significantly increased bone formation with markedly enhanced three-point bending strength compared to the negative control. Neovascularization and bone ingrowth, as well as highly organized bone formation, were also observed, showing the potential of iCMBA/HA in treating CBF.

Original languageEnglish (US)
Pages (from-to)387-398
Number of pages12
JournalJournal of Materials Chemistry B
Volume3
Issue number3
DOIs
StatePublished - Jan 21 2015

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Citric Acid
Bone
Durapatite
Hydroxyapatite
Tissue
Bone Substitutes
Bioceramics
Degradation
Bond strength (materials)
Stem cells
Biocompatibility
Bending strength
Strength of materials
Swelling
Repair

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Xie, Denghui ; Guo, Jinshan ; Mehdizadeh, M. Reza ; Tran, Richard T. ; Chen, Ruisong ; Sun, Dawei ; Qian, Guoying ; Jin, Dadi ; Bai, Xiaochun ; Yang, Jian. / Development of injectable citrate-based bioadhesive bone implants. In: Journal of Materials Chemistry B. 2015 ; Vol. 3, No. 3. pp. 387-398.
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Xie, D, Guo, J, Mehdizadeh, MR, Tran, RT, Chen, R, Sun, D, Qian, G, Jin, D, Bai, X & Yang, J 2015, 'Development of injectable citrate-based bioadhesive bone implants', Journal of Materials Chemistry B, vol. 3, no. 3, pp. 387-398. https://doi.org/10.1039/c4tb01498g

Development of injectable citrate-based bioadhesive bone implants. / Xie, Denghui; Guo, Jinshan; Mehdizadeh, M. Reza; Tran, Richard T.; Chen, Ruisong; Sun, Dawei; Qian, Guoying; Jin, Dadi; Bai, Xiaochun; Yang, Jian.

In: Journal of Materials Chemistry B, Vol. 3, No. 3, 21.01.2015, p. 387-398.

Research output: Contribution to journalArticle

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AU - Qian, Guoying

AU - Jin, Dadi

AU - Bai, Xiaochun

AU - Yang, Jian

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Xie D, Guo J, Mehdizadeh MR, Tran RT, Chen R, Sun D et al. Development of injectable citrate-based bioadhesive bone implants. Journal of Materials Chemistry B. 2015 Jan 21;3(3):387-398. https://doi.org/10.1039/c4tb01498g