Development of polymeric micro/nanostructures for gene delivery

Dania Alyounes, Christopher Yengo, Tim Doran, Qi Lu, Kenneth Gonsalves

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Pluronic® polymers were screened for binding affinity to oligoribonucleotides (ORN), also termed OLIGOS, using fluorescence techniques. F127 (Mw 12600 g/mole) proved to have the strongest binding affinity. Urethane linkages were introduced into F127 and two other Pluronics, F38 (Mw 4700 glmole) and F77 (Mw 6600 g/mole), to prepare polymers that were multiples of their respective initial molecular weights. A series of these polyurethane Pluronics were screened for binding affinity. Fluorescence studies showed a relationship between the molecular weight of the P0 units and the binding affinity in the structures of the newly synthesized poly Pluronics especially in the case of F77. Light scattering confirmed the binding affinity between the oligo and F77 polymers. Particle size analysis showed an exponential increase until the critical micelle concentration. Other analogs of these polymers also studied for their binding affinity were a poly(amino ester) and protective, interactive, noncondensing polymers (PINC).

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings - Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease
Pages41-48
Number of pages8
Publication statusPublished - Dec 1 2007
EventEngineered Nanoscale Materials for the Diagnosis and Treatment of Disease - 2007 MRS Spring Meeting - San Francisco, CA, United States
Duration: Apr 9 2007Apr 13 2007

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1019
ISSN (Print)0272-9172

Other

OtherEngineered Nanoscale Materials for the Diagnosis and Treatment of Disease - 2007 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period4/9/074/13/07

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Alyounes, D., Yengo, C., Doran, T., Lu, Q., & Gonsalves, K. (2007). Development of polymeric micro/nanostructures for gene delivery. In Materials Research Society Symposium Proceedings - Engineered Nanoscale Materials for the Diagnosis and Treatment of Disease (pp. 41-48). (Materials Research Society Symposium Proceedings; Vol. 1019).