Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum)

Anne P. Henderson, Timothy Lee King

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Shortnose sturgeon Acipenser brevirostrum is an endangered polyploid fish species for which no nuclear DNA markers previously existed. To address this need, 86 polysomic loci were developed and characterized in 20 A. brevirostrum from five river systems and eight members (parents and six progeny) of a captive-bred family. All markers proved to be polymorphic, polysomic, and demonstrated direct inheritance when tested in a captive family. Eleven loci were included in a range-wide survey of 561 fish sampled from 17 geographic collections. Allelic diversity at these markers ranged from 7 to 24 alleles/locus and averaged 16. 5 alleles/locus; sufficient diversity to produce unique multilocus genotypes. In the range-wide survey, a Mantel comparison of an ecological (1-Jaccard's) and genetic (Φ PT; an analog to F ST) distance metrics, identified a strong positive correlation (r = 0. 98, P < 0. 0001); suggesting Φ PT represents a viable metric for assessing genetic relatedness using this class of marker.

Original languageEnglish (US)
Pages (from-to)853-859
Number of pages7
JournalConservation Genetics Resources
Volume4
Issue number4
DOIs
StatePublished - Dec 1 2012

Fingerprint

Acipenser brevirostrum
Phylogeography
phylogeography
Microsatellite Repeats
Fishes
Alleles
microsatellite repeats
loci
Polyploidy
Genetic Markers
Rivers
Population
allele
Genotype
alleles
fish
relatedness
polyploidy
nuclear genome
river system

All Science Journal Classification (ASJC) codes

  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum)",
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Development of polysomic microsatellite markers for characterization of population structuring and phylogeography in the shortnose sturgeon (Acipenser brevirostrum). / Henderson, Anne P.; King, Timothy Lee.

In: Conservation Genetics Resources, Vol. 4, No. 4, 01.12.2012, p. 853-859.

Research output: Contribution to journalArticle

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