Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi

Sharon J. Anderson, Christine L. Stone, Martha Lucia Posada-Buitrago, Jeffrey L. Boore, Beena A. Neelam, Robert M. Stephens, Douglas G. Luster, Reid David Frederick, Kerry F. Pedley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alleles were observed for eight of the 24 loci. A preliminary screen with the closely related species, P. meibomiae, indicated that these primer pairs are specific to P. pachyrhizi.

Original languageEnglish (US)
Pages (from-to)1310-1312
Number of pages3
JournalMolecular Ecology Resources
Volume8
Issue number6
DOIs
StatePublished - Nov 1 2008

Fingerprint

Phakopsora pachyrhizi
soybean rust
rust disease
Soybeans
Microsatellite Repeats
soybean
allele
Fungi
fungus
microsatellite repeats
loci
fungi
Alleles
null alleles
pathogen
Fabaceae
Glycine max
legumes
soybeans
alleles

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Anderson, S. J., Stone, C. L., Posada-Buitrago, M. L., Boore, J. L., Neelam, B. A., Stephens, R. M., ... Pedley, K. F. (2008). Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi. Molecular Ecology Resources, 8(6), 1310-1312. https://doi.org/10.1111/j.1755-0998.2008.02272.x
Anderson, Sharon J. ; Stone, Christine L. ; Posada-Buitrago, Martha Lucia ; Boore, Jeffrey L. ; Neelam, Beena A. ; Stephens, Robert M. ; Luster, Douglas G. ; Frederick, Reid David ; Pedley, Kerry F. / Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi. In: Molecular Ecology Resources. 2008 ; Vol. 8, No. 6. pp. 1310-1312.
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Anderson, SJ, Stone, CL, Posada-Buitrago, ML, Boore, JL, Neelam, BA, Stephens, RM, Luster, DG, Frederick, RD & Pedley, KF 2008, 'Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi', Molecular Ecology Resources, vol. 8, no. 6, pp. 1310-1312. https://doi.org/10.1111/j.1755-0998.2008.02272.x

Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi. / Anderson, Sharon J.; Stone, Christine L.; Posada-Buitrago, Martha Lucia; Boore, Jeffrey L.; Neelam, Beena A.; Stephens, Robert M.; Luster, Douglas G.; Frederick, Reid David; Pedley, Kerry F.

In: Molecular Ecology Resources, Vol. 8, No. 6, 01.11.2008, p. 1310-1312.

Research output: Contribution to journalArticle

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Anderson SJ, Stone CL, Posada-Buitrago ML, Boore JL, Neelam BA, Stephens RM et al. Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi. Molecular Ecology Resources. 2008 Nov 1;8(6):1310-1312. https://doi.org/10.1111/j.1755-0998.2008.02272.x