Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi

Sharon J. Anderson, Christine L. Stone, Martha Lucia Posada-Buitrago, Jeffrey L. Boore, Beena A. Neelam, Robert M. Stephens, Douglas G. Luster, Reid D. Frederick, Kerry F. Pedley

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10 Scopus citations

Abstract

Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alleles were observed for eight of the 24 loci. A preliminary screen with the closely related species, P. meibomiae, indicated that these primer pairs are specific to P. pachyrhizi.

Original languageEnglish (US)
Pages (from-to)1310-1312
Number of pages3
JournalMolecular Ecology Resources
Volume8
Issue number6
DOIs
Publication statusPublished - Nov 1 2008

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Anderson, S. J., Stone, C. L., Posada-Buitrago, M. L., Boore, J. L., Neelam, B. A., Stephens, R. M., ... Pedley, K. F. (2008). Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi. Molecular Ecology Resources, 8(6), 1310-1312. https://doi.org/10.1111/j.1755-0998.2008.02272.x