Developmental changes in the adenine nucleotide translocase in the guinea pig

D. E. Hale, J. R. Williamson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Investigations of developmental changes in energy metabolism in guinea pig liver mitochondria showed that mitochondria from the newborn were well coupled, with respiratory control ratios and membrane energy potentials similar to those obtained with mitochondria from the 1-day-old and the adult. In contrast, there was a 3-fold increase in the rate of mitochondrial respiration and a 2-fold increase in adenine nucleotide content during the first 24 h of extrauterine life. There was no significant change in the ATP/ADP ratio and only a 30% increase in the uncoupled rate of respiration during the same time period. Titrations of the adenine nucleotide translocase with the specific inhibitor, carboxyatractyloside, showed that the newborn had only 50% of the adenine nucleotide translocase activity of the adult. Furthermore, by applying flux control theory to these inhibitor titrations, it was possible to demonstrate that the adenine nucleotide translocase exerted greater control over respiration in the newborn than in the adult, and at maximal rates of coupled respiration the translocase had a control strength of 0.98. The consequences of this finding on cellular energy metabolism are discussed in relation to adaptation of the newborn to extrauterine life.

Original languageEnglish (US)
Pages (from-to)8737-8742
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number14
StatePublished - Jan 1 1984

Fingerprint

ATP Translocases Mitochondrial ADP
Mitochondria
Respiratory Rate
Guinea Pigs
Titration
Energy Metabolism
Adenine Nucleotides
Liver Mitochondrion
Potential energy
Control theory
Liver
Membrane Potentials
Adenosine Diphosphate
Respiration
Adenosine Triphosphate
Fluxes
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{b415f9f4c7f34ae099f10a0fa2046ee1,
title = "Developmental changes in the adenine nucleotide translocase in the guinea pig",
abstract = "Investigations of developmental changes in energy metabolism in guinea pig liver mitochondria showed that mitochondria from the newborn were well coupled, with respiratory control ratios and membrane energy potentials similar to those obtained with mitochondria from the 1-day-old and the adult. In contrast, there was a 3-fold increase in the rate of mitochondrial respiration and a 2-fold increase in adenine nucleotide content during the first 24 h of extrauterine life. There was no significant change in the ATP/ADP ratio and only a 30{\%} increase in the uncoupled rate of respiration during the same time period. Titrations of the adenine nucleotide translocase with the specific inhibitor, carboxyatractyloside, showed that the newborn had only 50{\%} of the adenine nucleotide translocase activity of the adult. Furthermore, by applying flux control theory to these inhibitor titrations, it was possible to demonstrate that the adenine nucleotide translocase exerted greater control over respiration in the newborn than in the adult, and at maximal rates of coupled respiration the translocase had a control strength of 0.98. The consequences of this finding on cellular energy metabolism are discussed in relation to adaptation of the newborn to extrauterine life.",
author = "Hale, {D. E.} and Williamson, {J. R.}",
year = "1984",
month = "1",
day = "1",
language = "English (US)",
volume = "259",
pages = "8737--8742",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "14",

}

Developmental changes in the adenine nucleotide translocase in the guinea pig. / Hale, D. E.; Williamson, J. R.

In: Journal of Biological Chemistry, Vol. 259, No. 14, 01.01.1984, p. 8737-8742.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Developmental changes in the adenine nucleotide translocase in the guinea pig

AU - Hale, D. E.

AU - Williamson, J. R.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - Investigations of developmental changes in energy metabolism in guinea pig liver mitochondria showed that mitochondria from the newborn were well coupled, with respiratory control ratios and membrane energy potentials similar to those obtained with mitochondria from the 1-day-old and the adult. In contrast, there was a 3-fold increase in the rate of mitochondrial respiration and a 2-fold increase in adenine nucleotide content during the first 24 h of extrauterine life. There was no significant change in the ATP/ADP ratio and only a 30% increase in the uncoupled rate of respiration during the same time period. Titrations of the adenine nucleotide translocase with the specific inhibitor, carboxyatractyloside, showed that the newborn had only 50% of the adenine nucleotide translocase activity of the adult. Furthermore, by applying flux control theory to these inhibitor titrations, it was possible to demonstrate that the adenine nucleotide translocase exerted greater control over respiration in the newborn than in the adult, and at maximal rates of coupled respiration the translocase had a control strength of 0.98. The consequences of this finding on cellular energy metabolism are discussed in relation to adaptation of the newborn to extrauterine life.

AB - Investigations of developmental changes in energy metabolism in guinea pig liver mitochondria showed that mitochondria from the newborn were well coupled, with respiratory control ratios and membrane energy potentials similar to those obtained with mitochondria from the 1-day-old and the adult. In contrast, there was a 3-fold increase in the rate of mitochondrial respiration and a 2-fold increase in adenine nucleotide content during the first 24 h of extrauterine life. There was no significant change in the ATP/ADP ratio and only a 30% increase in the uncoupled rate of respiration during the same time period. Titrations of the adenine nucleotide translocase with the specific inhibitor, carboxyatractyloside, showed that the newborn had only 50% of the adenine nucleotide translocase activity of the adult. Furthermore, by applying flux control theory to these inhibitor titrations, it was possible to demonstrate that the adenine nucleotide translocase exerted greater control over respiration in the newborn than in the adult, and at maximal rates of coupled respiration the translocase had a control strength of 0.98. The consequences of this finding on cellular energy metabolism are discussed in relation to adaptation of the newborn to extrauterine life.

UR - http://www.scopus.com/inward/record.url?scp=0021233473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021233473&partnerID=8YFLogxK

M3 - Article

C2 - 6086610

AN - SCOPUS:0021233473

VL - 259

SP - 8737

EP - 8742

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 14

ER -