Developmental characterization of the gene for laminin α-chain in sea urchin embryos

Steve Benson, Laura Page, Eric Paul Ingersoll, Eric Rosenthal, Dungca Kate Dungca, Dawn Signor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We describe the isolation and characterization of a cDNA clone encoding a region of the carboxy terminal globular domain (G domain) of the α-1 chain of laminin from the sea urchin, Strongylocentrotus purpuratus. Sequence analysis indicates that the 1.3 kb cDNA (spLAM-α) encodes the complete G2 and G3 subdomains of sea urchin a-laminin. The 11 kb spLAM-α mRNA is present in the egg and declines slightly in abundance during development to the pluteus larva. The spLAM-α gene is also expressed in a variety of adult tissues. Whole mount in situ hybridization of gastrula stage embryos indicates that ectodermal and endodermal epithelia and mesenchyme cells contain the spLAM-α mRNA. Immunoprecipitation experiments using an antibody made to a recombinant fusion protein indicates spLAM-α protein is synthesized continuously from fertilization as a 420 kDa protein which accumulates from low levels in the egg to elevated levels in the pluteus larva. Light and electron microscopy identify spLAM-α as a component of the basal lamina. Blastocoelic microinjection of an antibody to recombinant spLAM-α perturbs gastrulation and skeleton formation by primary mesenchyme cells suggesting an important role for laminin in endodermal and mesodermal morphogenesis.

Original languageEnglish (US)
Pages (from-to)37-49
Number of pages13
JournalMechanisms of Development
Volume81
Issue number1-2
DOIs
StatePublished - Mar 1 1999

Fingerprint

Developmental Genes
Sea Urchins
Laminin
Mesoderm
Ovum
Larva
Embryonic Structures
Complementary DNA
Strongylocentrotus purpuratus
Recombinant Fusion Proteins
Gastrula
Gastrulation
Messenger RNA
Antibodies
Microinjections
Morphogenesis
Immunoprecipitation
Fertilization
Basement Membrane
Skeleton

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

Benson, Steve ; Page, Laura ; Ingersoll, Eric Paul ; Rosenthal, Eric ; Kate Dungca, Dungca ; Signor, Dawn. / Developmental characterization of the gene for laminin α-chain in sea urchin embryos. In: Mechanisms of Development. 1999 ; Vol. 81, No. 1-2. pp. 37-49.
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Developmental characterization of the gene for laminin α-chain in sea urchin embryos. / Benson, Steve; Page, Laura; Ingersoll, Eric Paul; Rosenthal, Eric; Kate Dungca, Dungca; Signor, Dawn.

In: Mechanisms of Development, Vol. 81, No. 1-2, 01.03.1999, p. 37-49.

Research output: Contribution to journalArticle

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