Developmental expression of calmodulin-dependent cyclic nucleotide phosphodiesterase in rat brain

Melvin Billingsley, Joseph W. Polli, Carey D. Balaban, Randall L. Kincaid

Research output: Contribution to journalArticle

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Abstract

The patterns of expression of calmodulin-dependent cyclic nucleotide phosphodiesterase (CaM-PDE) have been studied in developing and adult rat brain using affinity-purified polyclonal antibodies against CaM-PDE. An immunocytochemical map of adult brain regions expressing CaM-PDE, constructed from serial coronal brain sections, illustrated that CaM-PDE was expressed in specific neuronal subpopulations throughout the adult rat brain. Immunoblot analysis coupled with subcellular fractionation indicated that CaM-PDE was primarily localized to cytoplasmic fractions, with a small amount associated with synaptosomal membranes. Immunoblots from developing brain indicated that CaM-PDE expression increased dramatically during postnatal days 7-20 (PND 7-20); parallel increases in CaM-PDE enzyme activity occurred during this same time. Immunocytochemical studies indicated that several distinct patterns of CaM-PDE expression occurred during development. Neocortex showed low levels of CaM-PDE immunoreactivity in neuronal somata of layers III, V and VI on PND 4 that increased by PND 11: the adult somatodendritic pattern of immunoreactivity was observed by PND 60. Similar patterns were observed in cerebellar Purkinje cells, with somatodendritic staining observed by PND 12. By contrast, caudate-putamen, the inferior olive and the hypoglossal nuclei expressed high levels of CaM-PDE on PND 4, with levels considerably lower in the adult animal. The different patterns of expression suggest that in neocortex and cerebellum, CaM-PDE increases during the period of neuronal differentiation and active synaptogenesis, while in the caudate-putamen, inferior olive and hypoglossal nucleus, high levels may be required early in development.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalDevelopmental Brain Research
Volume53
Issue number2
DOIs
StatePublished - May 1 1990

Fingerprint

Cyclic Nucleotides
Phosphoric Diester Hydrolases
Calmodulin
Brain
Putamen
Neocortex
Purkinje Cells
Carisoprodol
Cerebellum
Staining and Labeling
Membranes
Antibodies
Enzymes

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Developmental Biology

Cite this

Billingsley, Melvin ; Polli, Joseph W. ; Balaban, Carey D. ; Kincaid, Randall L. / Developmental expression of calmodulin-dependent cyclic nucleotide phosphodiesterase in rat brain. In: Developmental Brain Research. 1990 ; Vol. 53, No. 2. pp. 253-263.
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Developmental expression of calmodulin-dependent cyclic nucleotide phosphodiesterase in rat brain. / Billingsley, Melvin; Polli, Joseph W.; Balaban, Carey D.; Kincaid, Randall L.

In: Developmental Brain Research, Vol. 53, No. 2, 01.05.1990, p. 253-263.

Research output: Contribution to journalArticle

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