Dextran sulfate sodium-induced colitis is associated with enhanced low molecular mass polypeptide 2 (LMP2) expression and is attenuated in LMP2 knockout mice

Leo R. Fitzpatrick, Vineeta Khare, Jeffrey S. Small, Walter A. Koltun

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Low molecular mass polypeptide 2 (LMP2) is an inducible proteasome subunit. Our goals were to examine LMP2 expression in mice with dextran sulfate sodium (DSS)-induced colitis and to evaluate colitis in LMP2 knockout (LMP2-/-) mice. Mice were given 2.5% DSS in the drinking water. On day 0, 2, 4, or 6 after DSS treatment, LMP2 expression was determined in the distal colon by western blot and immunohistochemistry. Parameters of colitis were measured in LMP2-/- mice or wild-type mice. LMP2 expression was enhanced in the colon of DSS-treated mice at all time points. Symptoms of DSS-induced colitis were always lower in LMP2-/- mice. Normalized histology scores and colonic IL-1β levels increased over the 6-day study period in wild-type mice. These parameters were significantly reduced in LMP2-/- mice that consumed DSS for 6 days. Enhanced LMP2 expression contributes to the pathogenesis of DSS-induced colitis in mice.

Original languageEnglish (US)
Pages (from-to)1269-1276
Number of pages8
JournalDigestive Diseases and Sciences
Volume51
Issue number7
DOIs
StatePublished - Jul 1 2006

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

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