Diagnosis and population analysis of Pythium species using AFLP fingerprinting

Carla D. Garzón, David Michael Geiser, Gary W. Moorman

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Accurate identification of Pythium species, the causal agents of Pythium root rot and dumping-off of seedlings, and characterization of populations within the species would greatly assist in selecting and implementing control strategies for these pathogens. Several molecular techniques offer methods for accurate and rapid identification of species, but provide little information about their populations. In this study, amplified fragment length polymorphism (AFLP) fingerprinting was used to characterize plant-pathogenic Pythium species and intraspecific populations. Species-diagnostic AFLP fingerprints for Pythium aphanidermatum, P. irregulare, and P. ultimum, and tentative fingerprints for six other species, were identified. Intraspecific distance analyses of P. aphanidermatum, P. ultimum, and P. irregulare revealed distinct patterns of intraspecific variation among the three species. P. aphanidermatum showed the smallest mean distance among isolates (15%), followed by P. ultimum (37%). P. irregulare had the largest mean distance among isolates (64%), which were divided into two populations with great genetic differentiation (FST = 0.2), suggesting the presence of a cryptic species boundary within this species.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalPlant Disease
Volume89
Issue number1
DOIs
StatePublished - Jan 2005

All Science Journal Classification (ASJC) codes

  • Plant Science

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