Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner

Troy D. Hubbard, Iain Alexander Murray, Robert G. Nichols, Kaitlyn Cassel, Michael Podolsky, Guray Kuzu, Yuan Tian, Philip B. Smith, Mary J. Kennett, Andrew David Patterson, Gary H. Perdew

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Consumption of broccoli mediates numerous chemo-protective benefits through the intake of phytochemicals, some of which modulate aryl hydrocarbon receptor (AHR) activity. Whether AHR activation is a critical aspect of the therapeutic potential of dietary broccoli is not known. Here we administered isocaloric diets, with or without supplementation of whole broccoli (15% w/w), to congenic mice expressing the high-affinity Ahr b/b or low-affinity Ahr d/d alleles, for 24 days and examined the effects on AHR activity, intestinal microbial community structure, inflammatory status, and response to chemically induced colitis. Cecal microbial community structure and metabolic potential were segregated according to host dietary and AHR status. Dietary broccoli associated with heightened intestinal AHR activity, decreased microbial abundance of the family Erysipelotrichaceae, and attenuation of colitis. In summary, broccoli consumption elicited an enhanced response in ligand-sensitive Ahr b/b mice, demonstrating that in part the beneficial aspects of dietary broccoli upon intestinal health are associated with heightened AHR activity.

Original languageEnglish (US)
Pages (from-to)685-698
Number of pages14
JournalJournal of Functional Foods
Volume37
DOIs
StatePublished - Oct 1 2017

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Aryl Hydrocarbon Receptors
Brassica
colitis
broccoli
Colitis
microbial communities
hydrocarbons
community structure
receptors
mice
Congenic Mice
Phytochemicals
phytopharmaceuticals
Alleles
Diet
Ligands
alleles
therapeutics
Health
diet

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

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title = "Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner",
abstract = "Consumption of broccoli mediates numerous chemo-protective benefits through the intake of phytochemicals, some of which modulate aryl hydrocarbon receptor (AHR) activity. Whether AHR activation is a critical aspect of the therapeutic potential of dietary broccoli is not known. Here we administered isocaloric diets, with or without supplementation of whole broccoli (15{\%} w/w), to congenic mice expressing the high-affinity Ahr b/b or low-affinity Ahr d/d alleles, for 24 days and examined the effects on AHR activity, intestinal microbial community structure, inflammatory status, and response to chemically induced colitis. Cecal microbial community structure and metabolic potential were segregated according to host dietary and AHR status. Dietary broccoli associated with heightened intestinal AHR activity, decreased microbial abundance of the family Erysipelotrichaceae, and attenuation of colitis. In summary, broccoli consumption elicited an enhanced response in ligand-sensitive Ahr b/b mice, demonstrating that in part the beneficial aspects of dietary broccoli upon intestinal health are associated with heightened AHR activity.",
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Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner. / Hubbard, Troy D.; Murray, Iain Alexander; Nichols, Robert G.; Cassel, Kaitlyn; Podolsky, Michael; Kuzu, Guray; Tian, Yuan; Smith, Philip B.; Kennett, Mary J.; Patterson, Andrew David; Perdew, Gary H.

In: Journal of Functional Foods, Vol. 37, 01.10.2017, p. 685-698.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Murray, Iain Alexander

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AU - Cassel, Kaitlyn

AU - Podolsky, Michael

AU - Kuzu, Guray

AU - Tian, Yuan

AU - Smith, Philip B.

AU - Kennett, Mary J.

AU - Patterson, Andrew David

AU - Perdew, Gary H.

PY - 2017/10/1

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