Different allele prevalence in the dihydrofolate reductase and dihydropteroate synthase genes in Plasmodium vivax populations from China

Miao Miao, Zhaoqing Yang, Long Cui, Jessica Ahlum, Yaming Huang, Liwang Cui

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Antifolate resistance in Plasmodium vivax is caused by point mutations in genes encoding dihydrofolate reductase (pvdhfr) and dihydropteroate synthase (pvdhps). In this study, we used direct sequencing to survey pvdhfr and pvdhps mutations in 122 clinical P. vivax isolates from a central and a southern province of China. For pvdhfr, 36.9% were wild-type, whereas mutations were detected at four codons (57, 58, 61, and 117). The S117N/T mutation was the most prevalent (48.4%), followed by the T61M mutation (18.9%). Six pvdhfr mutant alleles were found, ranging from 37.7% to 0.8%. The dramatically different pvdhfr allele frequencies between the two P. vivax populations might be caused by different drug histories or intrinsic difference between temperate and subtropical strains. In contrast, except polymorphisms within a repeat region, no resistance-conferring mutations were detected in pvdhps. Our result suggests that P vivax populations in China may be relatively susceptible to sulfadoxine-pyrimethamine.

Original languageEnglish (US)
Pages (from-to)1206-1211
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume83
Issue number6
DOIs
StatePublished - Dec 1 2010

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Virology
  • Infectious Diseases

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