Different repertoires of mouse T cells for bovine insulin presented by syngeneic and allogeneic cells

Norishisa Ishii, Jan Klein, Zoltan A. Nagy

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Splenic T cells were primed, after removal of alloreactive cells, to beef insulin on allogeneic antigen‐presenting cells (APC). The fine specificity of in vitro secondary response was tested in combinations H‐2b (responder) T cell‐H‐2k (nonresponder) APC, and vice versa, using separated chains of beef and pork insulin. The response in both combinations exhibited identical specificity patterns demonstrating that both responder and nonresponder APC could present the same array of insulin epitopes to allogeneic T cells. The determinants presented to allogeneic T cells include the A‐chain loop epitope and the B‐chain determinant(s) that were found to be immunogenic for H‐2b and H‐2d T cells, respectively, in the context of syngeneic major histocompatibility complex (HC) molecules. In addition, minor determinants were detected in the A chain outside the loop that are not immunogenic in syngeneic T cell‐APC combinations. Inhibition of T cell proliferation with monoclonal antibodies has shown that class II MHC molecules of the nonresponder (AαkAβk, EαkEβk) as well as those of the responder APC (AαbAβb) are equally capable of presenting virtually all insulin epitopes recognizable by T cells. The data, therefore, demonstrate that the selective recognition of different insulin epitopes observed in syngeneic or semisyngeneic T cell‐APC combinations does not result from determinant selection at the level of APC.

Original languageEnglish (US)
Pages (from-to)658-662
Number of pages5
JournalEuropean Journal of Immunology
Volume13
Issue number8
DOIs
StatePublished - 1983

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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