Differential Dependence on Host Cell Glycosaminoglycans for Infection of Epithelial Cells by High-Risk HPV Types

Linda Cruz, Craig Meyers

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Human papillomavirus (HPV) infection is the leading cause of cervical cancer world-wide. Here, we show that native HPV particles produced in a differentiated epithelium have developed different strategies to infect the host. Using biochemical inhibition assays and glycosaminoglycan (GAG)-negative cells, we show that of the four most common cancer-causing HPV types, HPV18, HPV31, and HPV45 are largely dependent on GAGs to initiate infection. In contrast, HPV16 can bind and enter through a GAG-independent mechanism. Infections of primary human keratinocytes, natural host cells for HPV infections, support our conclusions. Further, this renders the different virus types differentially susceptible to carrageenan, a microbicide targeting virus entry. Our data demonstrates that ordered maturation of papillomavirus particles in a differentiating epithelium may alter the virus entry mechanism. This study should facilitate a better understanding of the attachment and infection by the main oncogenic HPV types, and development of inhibitors of HPV infection.

Original languageEnglish (US)
Article numbere68379
JournalPloS one
Volume8
Issue number7
DOIs
StatePublished - Jul 4 2013

Fingerprint

Papillomaviridae
glycosaminoglycans
Glycosaminoglycans
Viruses
epithelial cells
Epithelial Cells
Papillomavirus Infections
Infection
infection
Virus Internalization
Carrageenan
cells
Anti-Infective Agents
Assays
viruses
Epithelium
epithelium
Human Development
uterine cervical neoplasms
Keratinocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

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Differential Dependence on Host Cell Glycosaminoglycans for Infection of Epithelial Cells by High-Risk HPV Types. / Cruz, Linda; Meyers, Craig.

In: PloS one, Vol. 8, No. 7, e68379, 04.07.2013.

Research output: Contribution to journalArticle

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