Differential destabilization of repetitive sequence hybrids in fluorescence in situ hybridization

J. C. Hozier, J. M. Scalzi, A. C. Clase, L. M. Davis, M. C. Liechty

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A method for painting a chromosome or chromosome region by fluorescence in situ hybridization (FISH) without blocking DNA is described. Both unique sequence and repetitive sequence components of a fluorescently labeled probe are hybridized under low-stringency conditions, but the chromosomes are washed in such a manner that repetitive sequences are differentially removed, while region-specific unique sequence fragments remain bound to the target chromosomes. We refer to this differential retention and removal of probe components as differential stability FISH.

Original languageEnglish (US)
Pages (from-to)60-63
Number of pages4
JournalCytogenetics and Cell Genetics
Volume83
Issue number1-2
DOIs
StatePublished - 1998

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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