Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell lines.

J. R. Emanuel, S. Garetz, L. Stone, Robert Levenson

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

We have analyzed Na+,K+-ATPase (EC 3.6.1.3) alpha- and beta-subunit mRNA expression in rat tissues and cell lines derived from the rat central nervous system. Substantial differences in the tissue and developmental specificity of expression were found for the genes encoding three isoforms of the alpha subunit. Transcripts of the alpha 1-subunit gene were detected in all tissues tested, whereas alpha 2- and alpha 3-subunit mRNA species were expressed predominantly in brain. The pattern of expression of beta-subunit mRNA also was complex and tissue specific but was distinct from that of any of the alpha-subunit mRNAs. Cell lines derived from the rat central nervous system and the pheochromocytoma PC12 expressed the mRNAs for all three alpha-subunit isoforms, whereas beta-subunit mRNA was detected only in PC12 cells. The distinct expression patterns of rat Na+,K+-ATPase mRNAs suggest that different members of the ATPase family may have specialized functions.

Original languageEnglish (US)
Pages (from-to)9030-9034
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number24
DOIs
StatePublished - Jan 1 1987

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Cell Line
Messenger RNA
Protein Isoforms
Central Nervous System
Organ Specificity
PC12 Cells
Pheochromocytoma
sodium-translocating ATPase
Genes
Adenosine Triphosphatases
Brain

All Science Journal Classification (ASJC) codes

  • General

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abstract = "We have analyzed Na+,K+-ATPase (EC 3.6.1.3) alpha- and beta-subunit mRNA expression in rat tissues and cell lines derived from the rat central nervous system. Substantial differences in the tissue and developmental specificity of expression were found for the genes encoding three isoforms of the alpha subunit. Transcripts of the alpha 1-subunit gene were detected in all tissues tested, whereas alpha 2- and alpha 3-subunit mRNA species were expressed predominantly in brain. The pattern of expression of beta-subunit mRNA also was complex and tissue specific but was distinct from that of any of the alpha-subunit mRNAs. Cell lines derived from the rat central nervous system and the pheochromocytoma PC12 expressed the mRNAs for all three alpha-subunit isoforms, whereas beta-subunit mRNA was detected only in PC12 cells. The distinct expression patterns of rat Na+,K+-ATPase mRNAs suggest that different members of the ATPase family may have specialized functions.",
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Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell lines. / Emanuel, J. R.; Garetz, S.; Stone, L.; Levenson, Robert.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 24, 01.01.1987, p. 9030-9034.

Research output: Contribution to journalArticle

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T1 - Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell lines.

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