Differential Expression of PRAMEL1, a Cancer/Testis Antigen, during Spermatogenesis in the Mouse

Bhavesh V. Mistry, Yaqi Zhao, Ti Cheng Chang, Hiroshi Yasue, Mitsuru Chiba, Jon Oatley, Francisco Javier Diaz, Wansheng Liu

Research output: Contribution to journalArticle

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Abstract

PRAME belongs to a group of cancer/testis antigens (CTAs) that are characterized by their restricted expression in normal gametogenic tissues and a variety of tumors. The PRAME family is one of the most amplified gene families in the mouse and other mammalian genomes. Members of the PRAME gene family encode leucine-rich repeat (LRR) proteins functioning as transcription regulators in cancer cells. However, the role of PRAME in normal gonads is unknown. The objective of this study is to characterize the temporal and spatial expression of the mouse Pramel1 gene, and to determine the cellular localization of the PRAMEL1 protein during the mouse spermatogenesis. Our results indicated that the mouse Pramel1 was expressed in testis only. The mRNA and protein expression level was low in the newborn testes, and gradually increased from 1- to 3-week-old testes, and then remained constant after three weeks of age. Immunofluorescent staining on testis sections with the mouse PRAMEL1 antibody revealed that PRAMEL1 was localized in the cytoplasm of spermatocytes and the acrosomal region of round, elongating and elongated spermatids. Further analyses on the testis squash preparation and spermatozoa at a subcellular level indicated that the protein localization patterns of PRAMEL1 were coordinated with morphological alterations during acrosome formation in spermatids, and were significantly different in connecting piece, middle piece and principal piece of the flagellum between testicular and epididymal spermatozoa. Collectively, our results suggest that PRAMEL1 may play a role in acrosome biogenesis and sperm motility.

Original languageEnglish (US)
Article numbere60611
JournalPloS one
Volume8
Issue number4
DOIs
StatePublished - Apr 2 2013

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Testicular Neoplasms
Spermatogenesis
spermatogenesis
Testis
testes
Genes
antigens
Antigens
neoplasms
mice
Acrosome
Spermatids
spermatids
acrosome
Spermatozoa
Proteins
Transcription
Cucurbita
spermatozoa
Spermatocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Mistry, Bhavesh V. ; Zhao, Yaqi ; Chang, Ti Cheng ; Yasue, Hiroshi ; Chiba, Mitsuru ; Oatley, Jon ; Diaz, Francisco Javier ; Liu, Wansheng. / Differential Expression of PRAMEL1, a Cancer/Testis Antigen, during Spermatogenesis in the Mouse. In: PloS one. 2013 ; Vol. 8, No. 4.
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Differential Expression of PRAMEL1, a Cancer/Testis Antigen, during Spermatogenesis in the Mouse. / Mistry, Bhavesh V.; Zhao, Yaqi; Chang, Ti Cheng; Yasue, Hiroshi; Chiba, Mitsuru; Oatley, Jon; Diaz, Francisco Javier; Liu, Wansheng.

In: PloS one, Vol. 8, No. 4, e60611, 02.04.2013.

Research output: Contribution to journalArticle

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AU - Mistry, Bhavesh V.

AU - Zhao, Yaqi

AU - Chang, Ti Cheng

AU - Yasue, Hiroshi

AU - Chiba, Mitsuru

AU - Oatley, Jon

AU - Diaz, Francisco Javier

AU - Liu, Wansheng

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AB - PRAME belongs to a group of cancer/testis antigens (CTAs) that are characterized by their restricted expression in normal gametogenic tissues and a variety of tumors. The PRAME family is one of the most amplified gene families in the mouse and other mammalian genomes. Members of the PRAME gene family encode leucine-rich repeat (LRR) proteins functioning as transcription regulators in cancer cells. However, the role of PRAME in normal gonads is unknown. The objective of this study is to characterize the temporal and spatial expression of the mouse Pramel1 gene, and to determine the cellular localization of the PRAMEL1 protein during the mouse spermatogenesis. Our results indicated that the mouse Pramel1 was expressed in testis only. The mRNA and protein expression level was low in the newborn testes, and gradually increased from 1- to 3-week-old testes, and then remained constant after three weeks of age. Immunofluorescent staining on testis sections with the mouse PRAMEL1 antibody revealed that PRAMEL1 was localized in the cytoplasm of spermatocytes and the acrosomal region of round, elongating and elongated spermatids. Further analyses on the testis squash preparation and spermatozoa at a subcellular level indicated that the protein localization patterns of PRAMEL1 were coordinated with morphological alterations during acrosome formation in spermatids, and were significantly different in connecting piece, middle piece and principal piece of the flagellum between testicular and epididymal spermatozoa. Collectively, our results suggest that PRAMEL1 may play a role in acrosome biogenesis and sperm motility.

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