Differential expression of the lactose transporter gene affects growth of Staphylococcus aureus in milk

M. V. Sharer, C. Su, N. V. Hegde, Bhushan M. Jayarao, L. M. Sordillo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, differential display polymerase chain reaction (PCR) was used to search for unique or enhanced expression of genes in prevalent bovine mastitis-causing Staphylococcus aureus strains. Comparison of a pair of prevalent and rare strains revealed the differential expression of several genes. The lactose-specific permease, enzyme II (EII), was highly expressed in the prevalent strain. This gene was selected for further study due to its potential influence on bacterial growth, because lactose is the primary carbohydrate in milk. Growth analysis illustrated that prevalent strains reach significantly higher growth densities sooner than rare strains. Quantitative competitive reverse transcription PCR (QC RT-PCR) revealed increased EII mRNA expression in prevalent strains as compared to rare strains. Mutation of the EII gene resulted in abrogated growth and decreased EII mRNA expression in media containing lactose. These data suggest that increased EII expression may facilitate the pathogenesis of S. aureus mastitis by enhancing growth. This study is the first to implicate EII as a potential virulence factor in mastitis, and therefore may be useful in the development of novel therapeutic strategies against S. aureus mastitis.

Original languageEnglish (US)
Pages (from-to)2373-2381
Number of pages9
JournalJournal of dairy science
Volume86
Issue number7
DOIs
StatePublished - Jan 1 2003

Fingerprint

Lactose
lactose
transporters
Staphylococcus aureus
Milk
milk
Mastitis
Enzymes
Growth
Genes
enzymes
genes
mastitis
Bovine Mastitis
Gene Expression
Polymerase Chain Reaction
Messenger RNA
gene expression
Virulence Factors
bovine mastitis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Sharer, M. V. ; Su, C. ; Hegde, N. V. ; Jayarao, Bhushan M. ; Sordillo, L. M. / Differential expression of the lactose transporter gene affects growth of Staphylococcus aureus in milk. In: Journal of dairy science. 2003 ; Vol. 86, No. 7. pp. 2373-2381.
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Differential expression of the lactose transporter gene affects growth of Staphylococcus aureus in milk. / Sharer, M. V.; Su, C.; Hegde, N. V.; Jayarao, Bhushan M.; Sordillo, L. M.

In: Journal of dairy science, Vol. 86, No. 7, 01.01.2003, p. 2373-2381.

Research output: Contribution to journalArticle

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