Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit

S. Fiori, B. Scherm, Q. Migheli, Robert E. Farrell, Jr., M. Budroni, M. Wisniewski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A biofilm-forming strain of Pichia fermentans was found to be a very strong antagonist against brown rot and grey mold in artificially wounded apple fruit when co-inoculated with either Monilinia fructicola or Botrytis cinerea, respectively. The same strain of yeast, however, was an aggressive pathogen when inoculated on peach fruit, causing rot of fruit tissues, even in the absence of other pathogens. Optical and scanning electron microscopy showed that P. fermentans produces only yeast-like shaped cells during colonization of apple tissue, while exhibiting pseudohyphal growth on peach tissue. A rapid subtractive hybridization approach (RaSH) was used to identify differentially expressed genes in the pathogenic form of P. fermentans by comparing the cDNA of P. fermentans sampled after 24 hours growth on apple with the cDNA of the same strain grown 24 hours on peach fruit. A total of 450 clones were analysed by a reverse Northern Blotting technique, yielding some fragments which were significantly expressed on peach but less on apple tissue. These sequences were compared to the available genome sequences of another dimorphic yeast, Candida albicans, and homologous genes were identified. The relationship between these genes, dimorphism, and pathogenicity will be discussed.

Original languageEnglish (US)
Title of host publicationInternational Symposium on Biological Control of Postharvest Diseases
Subtitle of host publicationChallenges and Opportunities
EditorsM. Wisniewski, S. Droby
Pages103-105
Number of pages3
StatePublished - Aug 31 2011

Publication series

NameActa Horticulturae
Volume905
ISSN (Print)0567-7572

Fingerprint

Pichia fermentans
peaches
apples
gene expression
fruits
yeasts
Monilinia fructicola
suppression subtractive hybridization
genes
pathogens
Botrytis cinerea
dimorphism
Candida albicans
Northern blotting
biofilm
antagonists
scanning electron microscopy
pathogenicity
clones
genome

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Fiori, S., Scherm, B., Migheli, Q., Farrell, Jr., R. E., Budroni, M., & Wisniewski, M. (2011). Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit. In M. Wisniewski, & S. Droby (Eds.), International Symposium on Biological Control of Postharvest Diseases: Challenges and Opportunities (pp. 103-105). (Acta Horticulturae; Vol. 905).
Fiori, S. ; Scherm, B. ; Migheli, Q. ; Farrell, Jr., Robert E. ; Budroni, M. ; Wisniewski, M. / Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit. International Symposium on Biological Control of Postharvest Diseases: Challenges and Opportunities. editor / M. Wisniewski ; S. Droby. 2011. pp. 103-105 (Acta Horticulturae).
@inproceedings{bf4928f2407f474da8903b7d5a38dbfd,
title = "Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit",
abstract = "A biofilm-forming strain of Pichia fermentans was found to be a very strong antagonist against brown rot and grey mold in artificially wounded apple fruit when co-inoculated with either Monilinia fructicola or Botrytis cinerea, respectively. The same strain of yeast, however, was an aggressive pathogen when inoculated on peach fruit, causing rot of fruit tissues, even in the absence of other pathogens. Optical and scanning electron microscopy showed that P. fermentans produces only yeast-like shaped cells during colonization of apple tissue, while exhibiting pseudohyphal growth on peach tissue. A rapid subtractive hybridization approach (RaSH) was used to identify differentially expressed genes in the pathogenic form of P. fermentans by comparing the cDNA of P. fermentans sampled after 24 hours growth on apple with the cDNA of the same strain grown 24 hours on peach fruit. A total of 450 clones were analysed by a reverse Northern Blotting technique, yielding some fragments which were significantly expressed on peach but less on apple tissue. These sequences were compared to the available genome sequences of another dimorphic yeast, Candida albicans, and homologous genes were identified. The relationship between these genes, dimorphism, and pathogenicity will be discussed.",
author = "S. Fiori and B. Scherm and Q. Migheli and {Farrell, Jr.}, {Robert E.} and M. Budroni and M. Wisniewski",
year = "2011",
month = "8",
day = "31",
language = "English (US)",
isbn = "9789066053571",
series = "Acta Horticulturae",
pages = "103--105",
editor = "M. Wisniewski and S. Droby",
booktitle = "International Symposium on Biological Control of Postharvest Diseases",

}

Fiori, S, Scherm, B, Migheli, Q, Farrell, Jr., RE, Budroni, M & Wisniewski, M 2011, Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit. in M Wisniewski & S Droby (eds), International Symposium on Biological Control of Postharvest Diseases: Challenges and Opportunities. Acta Horticulturae, vol. 905, pp. 103-105.

Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit. / Fiori, S.; Scherm, B.; Migheli, Q.; Farrell, Jr., Robert E.; Budroni, M.; Wisniewski, M.

International Symposium on Biological Control of Postharvest Diseases: Challenges and Opportunities. ed. / M. Wisniewski; S. Droby. 2011. p. 103-105 (Acta Horticulturae; Vol. 905).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit

AU - Fiori, S.

AU - Scherm, B.

AU - Migheli, Q.

AU - Farrell, Jr., Robert E.

AU - Budroni, M.

AU - Wisniewski, M.

PY - 2011/8/31

Y1 - 2011/8/31

N2 - A biofilm-forming strain of Pichia fermentans was found to be a very strong antagonist against brown rot and grey mold in artificially wounded apple fruit when co-inoculated with either Monilinia fructicola or Botrytis cinerea, respectively. The same strain of yeast, however, was an aggressive pathogen when inoculated on peach fruit, causing rot of fruit tissues, even in the absence of other pathogens. Optical and scanning electron microscopy showed that P. fermentans produces only yeast-like shaped cells during colonization of apple tissue, while exhibiting pseudohyphal growth on peach tissue. A rapid subtractive hybridization approach (RaSH) was used to identify differentially expressed genes in the pathogenic form of P. fermentans by comparing the cDNA of P. fermentans sampled after 24 hours growth on apple with the cDNA of the same strain grown 24 hours on peach fruit. A total of 450 clones were analysed by a reverse Northern Blotting technique, yielding some fragments which were significantly expressed on peach but less on apple tissue. These sequences were compared to the available genome sequences of another dimorphic yeast, Candida albicans, and homologous genes were identified. The relationship between these genes, dimorphism, and pathogenicity will be discussed.

AB - A biofilm-forming strain of Pichia fermentans was found to be a very strong antagonist against brown rot and grey mold in artificially wounded apple fruit when co-inoculated with either Monilinia fructicola or Botrytis cinerea, respectively. The same strain of yeast, however, was an aggressive pathogen when inoculated on peach fruit, causing rot of fruit tissues, even in the absence of other pathogens. Optical and scanning electron microscopy showed that P. fermentans produces only yeast-like shaped cells during colonization of apple tissue, while exhibiting pseudohyphal growth on peach tissue. A rapid subtractive hybridization approach (RaSH) was used to identify differentially expressed genes in the pathogenic form of P. fermentans by comparing the cDNA of P. fermentans sampled after 24 hours growth on apple with the cDNA of the same strain grown 24 hours on peach fruit. A total of 450 clones were analysed by a reverse Northern Blotting technique, yielding some fragments which were significantly expressed on peach but less on apple tissue. These sequences were compared to the available genome sequences of another dimorphic yeast, Candida albicans, and homologous genes were identified. The relationship between these genes, dimorphism, and pathogenicity will be discussed.

UR - http://www.scopus.com/inward/record.url?scp=80053387039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053387039&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:80053387039

SN - 9789066053571

T3 - Acta Horticulturae

SP - 103

EP - 105

BT - International Symposium on Biological Control of Postharvest Diseases

A2 - Wisniewski, M.

A2 - Droby, S.

ER -

Fiori S, Scherm B, Migheli Q, Farrell, Jr. RE, Budroni M, Wisniewski M. Differential gene expression during the pathogenic interaction between Pichia fermentans and peach fruit. In Wisniewski M, Droby S, editors, International Symposium on Biological Control of Postharvest Diseases: Challenges and Opportunities. 2011. p. 103-105. (Acta Horticulturae).