Differential gene expression to investigate the effect of (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis

Dacheng Ren, Laura A. Bedzyk, Peter Setlow, Dacre F. England, Staffan Kjelleberg, Stuart M. Thomas, Rick W. Ye, Thomas Keith Wood

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

(5Z)-4-Bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone (furanone) from the red marine alga Delisea pulchra was found previously to inhibit the growth, swarming, and biofilm formation of gram-positive bacteria. Using the gram-positive bacterium Bacillus subtilis as a test organism, we observed cell killing by 20 μg of furanone per ml, while 5 μg of furanone per ml inhibited growth approximately twofold without killing the cells. To discover the mechanism of this inhibition on a genetic level and to investigate furanone as a novel antibiotic, full-genome DNA microarrays were used to analyze the gene expression profiles of A. subtilis grown with and without 5 μg of furanone per ml. This agent induced 92 genes more than fivefold (P < 0.05) and repressed 15 genes more than fivefold (P < 0.05). The induced genes include genes involved in stress responses (such as the class III heat shock genes clpC, clpE, and ctsR and the class I heat shock genes groES, but no class II or IV heat shock genes), fatty acid biosynthesis, lichenan degradation, transport, and metabolism, as well as 59 genes with unknown functions. The microarray results for four genes were confirmed by RNA dot blotting. Mutation of a stress response gene, clpC, caused B. subtilis to be much more sensitive to 5 μg of furanone per ml (there was NO growth in 8 h, while the wild-type strain grew to the stationary phase in 8 h) and confirmed the importance of the induction of this gene as identified by the microarray analysis.

Original languageEnglish (US)
Pages (from-to)4941-4949
Number of pages9
JournalApplied and environmental microbiology
Volume70
Issue number8
DOIs
StatePublished - Aug 1 2004

Fingerprint

Bacillus subtilis
gene expression
Gene Expression
gene
Genes
genes
heat shock
heat stress
Shock
Hot Temperature
Gram-positive bacteria
Gram-Positive Bacteria
algae
stress response
Delisea
4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone
effect
Growth
swarming
Rhodophyta

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Ren, Dacheng ; Bedzyk, Laura A. ; Setlow, Peter ; England, Dacre F. ; Kjelleberg, Staffan ; Thomas, Stuart M. ; Ye, Rick W. ; Wood, Thomas Keith. / Differential gene expression to investigate the effect of (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis. In: Applied and environmental microbiology. 2004 ; Vol. 70, No. 8. pp. 4941-4949.
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abstract = "(5Z)-4-Bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone (furanone) from the red marine alga Delisea pulchra was found previously to inhibit the growth, swarming, and biofilm formation of gram-positive bacteria. Using the gram-positive bacterium Bacillus subtilis as a test organism, we observed cell killing by 20 μg of furanone per ml, while 5 μg of furanone per ml inhibited growth approximately twofold without killing the cells. To discover the mechanism of this inhibition on a genetic level and to investigate furanone as a novel antibiotic, full-genome DNA microarrays were used to analyze the gene expression profiles of A. subtilis grown with and without 5 μg of furanone per ml. This agent induced 92 genes more than fivefold (P < 0.05) and repressed 15 genes more than fivefold (P < 0.05). The induced genes include genes involved in stress responses (such as the class III heat shock genes clpC, clpE, and ctsR and the class I heat shock genes groES, but no class II or IV heat shock genes), fatty acid biosynthesis, lichenan degradation, transport, and metabolism, as well as 59 genes with unknown functions. The microarray results for four genes were confirmed by RNA dot blotting. Mutation of a stress response gene, clpC, caused B. subtilis to be much more sensitive to 5 μg of furanone per ml (there was NO growth in 8 h, while the wild-type strain grew to the stationary phase in 8 h) and confirmed the importance of the induction of this gene as identified by the microarray analysis.",
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Differential gene expression to investigate the effect of (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis. / Ren, Dacheng; Bedzyk, Laura A.; Setlow, Peter; England, Dacre F.; Kjelleberg, Staffan; Thomas, Stuart M.; Ye, Rick W.; Wood, Thomas Keith.

In: Applied and environmental microbiology, Vol. 70, No. 8, 01.08.2004, p. 4941-4949.

Research output: Contribution to journalArticle

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T1 - Differential gene expression to investigate the effect of (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis

AU - Ren, Dacheng

AU - Bedzyk, Laura A.

AU - Setlow, Peter

AU - England, Dacre F.

AU - Kjelleberg, Staffan

AU - Thomas, Stuart M.

AU - Ye, Rick W.

AU - Wood, Thomas Keith

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