Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection

Anna M. Seekatz, Aruna Panda, David A. Rasko, Franklin R. Toapanta, Emiley A. Eloe-Fadrosh, Abdul Q. Khan, Zhenqiu Liu, Steven T. Shipley, Louis J. DeTolla, Marcelo B. Sztein, Claire M. Fraser

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Little is known about the role of gut microbiota in response to live oral vaccines against enteric pathogens. We examined the effect of immunization with an oral live-attenuated Shigella dysenteriae 1 vaccine and challenge with wild-type S. dysenteriae 1 on the fecal microbiota of cynomolgus macaques using 16 S rRNA analysis of fecal samples. Multi-dimensional cluster analysis identified different bacterial community types within macaques from geographically distinct locations. The fecal microbiota of Mauritian macaques, observed to be genetically distinct, harbored a high-diversity community and responded differently to Shigella immunization, as well as challenge compared to the microbiota in non-Mauritian macaques. While both macaque populations exhibited anti-Shigella antibody responses, clinical shigellosis was observed only among non-Mauritian macaques. These studies highlight the importance of further investigation into the possible protective role of the microbiota against enteric pathogens and consideration of host genetic backgrounds in conducting vaccine studies.

Original languageEnglish (US)
Article numbere64212
JournalPloS one
Volume8
Issue number6
DOIs
StatePublished - Jun 5 2013

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Shigella
Macaca fascicularis
Macaca
intestinal microorganisms
Immunization
Vaccines
Microbiota
Pathogens
Shigella dysenteriae
Infection
infection
vaccines
Cluster analysis
mouth
immunization
shigellosis
pathogens
Bacillary Dysentery
bacterial communities
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Seekatz, A. M., Panda, A., Rasko, D. A., Toapanta, F. R., Eloe-Fadrosh, E. A., Khan, A. Q., ... Fraser, C. M. (2013). Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection. PloS one, 8(6), [e64212]. https://doi.org/10.1371/journal.pone.0064212
Seekatz, Anna M. ; Panda, Aruna ; Rasko, David A. ; Toapanta, Franklin R. ; Eloe-Fadrosh, Emiley A. ; Khan, Abdul Q. ; Liu, Zhenqiu ; Shipley, Steven T. ; DeTolla, Louis J. ; Sztein, Marcelo B. ; Fraser, Claire M. / Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection. In: PloS one. 2013 ; Vol. 8, No. 6.
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Seekatz, AM, Panda, A, Rasko, DA, Toapanta, FR, Eloe-Fadrosh, EA, Khan, AQ, Liu, Z, Shipley, ST, DeTolla, LJ, Sztein, MB & Fraser, CM 2013, 'Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection', PloS one, vol. 8, no. 6, e64212. https://doi.org/10.1371/journal.pone.0064212

Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection. / Seekatz, Anna M.; Panda, Aruna; Rasko, David A.; Toapanta, Franklin R.; Eloe-Fadrosh, Emiley A.; Khan, Abdul Q.; Liu, Zhenqiu; Shipley, Steven T.; DeTolla, Louis J.; Sztein, Marcelo B.; Fraser, Claire M.

In: PloS one, Vol. 8, No. 6, e64212, 05.06.2013.

Research output: Contribution to journalArticle

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Seekatz AM, Panda A, Rasko DA, Toapanta FR, Eloe-Fadrosh EA, Khan AQ et al. Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection. PloS one. 2013 Jun 5;8(6). e64212. https://doi.org/10.1371/journal.pone.0064212