Differential roles of C4 and βC1 in mediating suppression of post-transcriptional gene silencing: Evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirus

P. Gopal, P. Pravin Kumar, B. Sinilal, J. Jose, A. Kasin Yadunandam, R. Usha

Research output: Contribution to journalArticle

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Abstract

Bhendi yellow vein mosaic disease (BYVMD) is caused by the association of a DNA β satellite with a begomovirus component. The begomovirus component has two promoters, one in the virion sense (V-sense) and the other in the complementary sense (C-sense) in the intergenic region (IR). To study the promoter activities of V-sense and C-sense promoters, mGFP gene fusion was made downstream to the promoters. Transient and stable expressions in N. benthamiana leaves showed significant GFP expression under C-sense promoter whereas the expression under the V-sense promoter was very weak in the absence of the transactivator C2. Untransformed N. benthamiana plants were agroinfiltrated with binary vector constructs containing V-sense-GFP alone or along with C1, C2, C4, V1, V2 or βC1 (in both sense and antisense orientations) to understand the roles of these gene products in transactivation and/or suppression of post-transcriptional gene silencing (PTGS). The results showed strong suppression of gene silencing activities for C4 and βC1 but a weak activity for C2. The suppression activities were also confirmed using gfp-silenced GFP16c/GFPi plants by agroinfiltration and agroinoculation. The expression of C4 and βC1 as transgenes produced abnormal phenotypic growth compared to the other viral genes mentioned above, further supporting their suppressor function.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalVirus Research
Volume123
Issue number1
DOIs
StatePublished - Jan 1 2007

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Begomovirus
Mosaic Viruses
RNA Interference
Virion
Transcriptional Activation
Veins
Satellite DNA
Intergenic DNA
Trans-Activators
Viral Genes
Gene Fusion
Gene Silencing
Transgenes
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

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title = "Differential roles of C4 and βC1 in mediating suppression of post-transcriptional gene silencing: Evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirus",
abstract = "Bhendi yellow vein mosaic disease (BYVMD) is caused by the association of a DNA β satellite with a begomovirus component. The begomovirus component has two promoters, one in the virion sense (V-sense) and the other in the complementary sense (C-sense) in the intergenic region (IR). To study the promoter activities of V-sense and C-sense promoters, mGFP gene fusion was made downstream to the promoters. Transient and stable expressions in N. benthamiana leaves showed significant GFP expression under C-sense promoter whereas the expression under the V-sense promoter was very weak in the absence of the transactivator C2. Untransformed N. benthamiana plants were agroinfiltrated with binary vector constructs containing V-sense-GFP alone or along with C1, C2, C4, V1, V2 or βC1 (in both sense and antisense orientations) to understand the roles of these gene products in transactivation and/or suppression of post-transcriptional gene silencing (PTGS). The results showed strong suppression of gene silencing activities for C4 and βC1 but a weak activity for C2. The suppression activities were also confirmed using gfp-silenced GFP16c/GFPi plants by agroinfiltration and agroinoculation. The expression of C4 and βC1 as transgenes produced abnormal phenotypic growth compared to the other viral genes mentioned above, further supporting their suppressor function.",
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Differential roles of C4 and βC1 in mediating suppression of post-transcriptional gene silencing : Evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirus. / Gopal, P.; Pravin Kumar, P.; Sinilal, B.; Jose, J.; Kasin Yadunandam, A.; Usha, R.

In: Virus Research, Vol. 123, No. 1, 01.01.2007, p. 9-18.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differential roles of C4 and βC1 in mediating suppression of post-transcriptional gene silencing

T2 - Evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirus

AU - Gopal, P.

AU - Pravin Kumar, P.

AU - Sinilal, B.

AU - Jose, J.

AU - Kasin Yadunandam, A.

AU - Usha, R.

PY - 2007/1/1

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AB - Bhendi yellow vein mosaic disease (BYVMD) is caused by the association of a DNA β satellite with a begomovirus component. The begomovirus component has two promoters, one in the virion sense (V-sense) and the other in the complementary sense (C-sense) in the intergenic region (IR). To study the promoter activities of V-sense and C-sense promoters, mGFP gene fusion was made downstream to the promoters. Transient and stable expressions in N. benthamiana leaves showed significant GFP expression under C-sense promoter whereas the expression under the V-sense promoter was very weak in the absence of the transactivator C2. Untransformed N. benthamiana plants were agroinfiltrated with binary vector constructs containing V-sense-GFP alone or along with C1, C2, C4, V1, V2 or βC1 (in both sense and antisense orientations) to understand the roles of these gene products in transactivation and/or suppression of post-transcriptional gene silencing (PTGS). The results showed strong suppression of gene silencing activities for C4 and βC1 but a weak activity for C2. The suppression activities were also confirmed using gfp-silenced GFP16c/GFPi plants by agroinfiltration and agroinoculation. The expression of C4 and βC1 as transgenes produced abnormal phenotypic growth compared to the other viral genes mentioned above, further supporting their suppressor function.

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