Differential YAP expression in glioma cells induces cell competition and promotes tumorigenesis

Zhijun Liu, Patricia P. Yee, Yiju Wei, Zhenqiu Liu, Yuka Imamura, Wei Li

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Intratumor heterogeneity associates with cancer progression and may account for a substantial portion of therapeutic resistance. Although extensive studies have focused on the origin of the heterogeneity, biological interactions between heterogeneous malignant cells within a tumor are largely unexplored. Glioblastoma (GBM) is the most aggressive primary brain tumor. Here, we found that the expression of Yes-associated protein (YAP, also known as YAP1) is intratumorally heterogeneous in GBM. In a xenograft mouse model, differential YAP expression in glioma cells promotes tumorigenesis and leads to clonal dominance by cells expressing more YAP. Such clonal dominance also occurs in vitro when cells reach confluence in the two-dimensional culture condition or grow into tumor spheroids. During this process, growth of the dominant cell population is enhanced. In the tumor spheroid, such enhanced growth is accompanied by increased apoptosis in cells expressing less YAP. The cellular interaction during clonal dominance appears to be reminiscent of cell competition. RNA-seq analysis suggests that this interaction induces expression of tumorigenic genes, which may contribute to the enhanced tumor growth. These results suggest that tumorigenesis benefits from competitive interactions between heterogeneous tumor cells.

Original languageEnglish (US)
Article numberjcs225714
JournalJournal of Cell Science
Volume132
Issue number5
DOIs
StatePublished - Mar 1 2019

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Glioma
Carcinogenesis
Neoplasms
Glioblastoma
Growth
Heterografts
Brain Neoplasms
RNA
Apoptosis
Gene Expression
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

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title = "Differential YAP expression in glioma cells induces cell competition and promotes tumorigenesis",
abstract = "Intratumor heterogeneity associates with cancer progression and may account for a substantial portion of therapeutic resistance. Although extensive studies have focused on the origin of the heterogeneity, biological interactions between heterogeneous malignant cells within a tumor are largely unexplored. Glioblastoma (GBM) is the most aggressive primary brain tumor. Here, we found that the expression of Yes-associated protein (YAP, also known as YAP1) is intratumorally heterogeneous in GBM. In a xenograft mouse model, differential YAP expression in glioma cells promotes tumorigenesis and leads to clonal dominance by cells expressing more YAP. Such clonal dominance also occurs in vitro when cells reach confluence in the two-dimensional culture condition or grow into tumor spheroids. During this process, growth of the dominant cell population is enhanced. In the tumor spheroid, such enhanced growth is accompanied by increased apoptosis in cells expressing less YAP. The cellular interaction during clonal dominance appears to be reminiscent of cell competition. RNA-seq analysis suggests that this interaction induces expression of tumorigenic genes, which may contribute to the enhanced tumor growth. These results suggest that tumorigenesis benefits from competitive interactions between heterogeneous tumor cells.",
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Differential YAP expression in glioma cells induces cell competition and promotes tumorigenesis. / Liu, Zhijun; Yee, Patricia P.; Wei, Yiju; Liu, Zhenqiu; Imamura, Yuka; Li, Wei.

In: Journal of Cell Science, Vol. 132, No. 5, jcs225714, 01.03.2019.

Research output: Contribution to journalArticle

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AU - Liu, Zhijun

AU - Yee, Patricia P.

AU - Wei, Yiju

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AU - Imamura, Yuka

AU - Li, Wei

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