Differentiating Pseudomonas sp. strain ADP cells in suspensions and biofilms using Raman spectroscopy and scanning electron microscopy

Victoria A. Henry, Julie L.P. Jessop, Tonya Peeples

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High quality spectra of Pseudomonas sp. strain ADP in the planktonic and biofilm state were obtained using Raman microspectroscopy. These spectra enabled the identification of key differences between free and biofilm cells in the fingerprint region of Raman spectra in the nucleic acid, carbohydrate, and protein regions. Scanning electron microscopy (SEM) enabled detailed visualization of ADP biofilm with confirmation of associated extracellular matrix structure. Following extraction and Raman analysis of extracellular polymeric substances, Raman spectral differences between free and biofilm cells were largely attributed to the contribution of extracellular matrix components produced in mature biofilms. Raman spectroscopy complemented with SEM proves to be useful in distinguishing physiological properties among cells of the same species. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)1441-1449
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume409
Issue number5
DOIs
StatePublished - Jan 1 2017

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Raman Spectrum Analysis
Biofilms
Pseudomonas
Electron Scanning Microscopy
Adenosine Diphosphate
Raman spectroscopy
Suspensions
Scanning electron microscopy
Extracellular Matrix
Dermatoglyphics
Nucleic Acids
Raman scattering
Visualization
Carbohydrates
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

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abstract = "High quality spectra of Pseudomonas sp. strain ADP in the planktonic and biofilm state were obtained using Raman microspectroscopy. These spectra enabled the identification of key differences between free and biofilm cells in the fingerprint region of Raman spectra in the nucleic acid, carbohydrate, and protein regions. Scanning electron microscopy (SEM) enabled detailed visualization of ADP biofilm with confirmation of associated extracellular matrix structure. Following extraction and Raman analysis of extracellular polymeric substances, Raman spectral differences between free and biofilm cells were largely attributed to the contribution of extracellular matrix components produced in mature biofilms. Raman spectroscopy complemented with SEM proves to be useful in distinguishing physiological properties among cells of the same species. [Figure not available: see fulltext.]",
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Differentiating Pseudomonas sp. strain ADP cells in suspensions and biofilms using Raman spectroscopy and scanning electron microscopy. / Henry, Victoria A.; Jessop, Julie L.P.; Peeples, Tonya.

In: Analytical and Bioanalytical Chemistry, Vol. 409, No. 5, 01.01.2017, p. 1441-1449.

Research output: Contribution to journalArticle

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