Differentiation of y79 retinoblastoma cells with pigment epithelial-derived factor and interphotoreceptor matrix wash: Effects on tumorigenicity

Gail M. Seigel, Joyce Tombran-Tink, S. Patricia Becerra, Gerald J. Chader, David A. Diloreto, Constancia Del Cerro, Eliot S. Lazar, Manuel Del Cerro

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

We investigated the in vivo differentiation potential of Y79 human retinoblastoma cells following pre-treatment with two novel neurotrophic agents: PEDF (human recombinant pigmented-epithelial derived factor) or IPM (interphotoreceptor matrix) wash. These agents were able to induce a significant degree of morphological differentiation in vitro. However, 48 days after subretinal transplantation of pre-treated cells, massive tumor formation was apparent. In contrast, Y79 cells pre-treated with retinoic acid/sodium butyrate, which attain a lesser degree of morphological differentiation, did not produce tumors over a 30 to 60 day-survival time (del Cerro et al., Brain Research, 12-22, 1992). We conclude that for PEDF and IPM, the degree of in vitro differentiation and the degree of mitotic arrest are independent features.

Original languageEnglish (US)
Pages (from-to)289-297
Number of pages9
JournalGrowth Factors
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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