Differentiation of y79 retinoblastoma cells with pigment epithelial-derived factor and interphotoreceptor matrix wash: Effects on tumorigenicity

Gail M. Seigel, Joyce Tombran-Tink, S. Patricia Becerra, Gerald J. Chader, David A. Diloreto, Constancia Del Cerro, Eliot S. Lazar, Manuel Del Cerro

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We investigated the in vivo differentiation potential of Y79 human retinoblastoma cells following pre-treatment with two novel neurotrophic agents: PEDF (human recombinant pigmented-epithelial derived factor) or IPM (interphotoreceptor matrix) wash. These agents were able to induce a significant degree of morphological differentiation in vitro. However, 48 days after subretinal transplantation of pre-treated cells, massive tumor formation was apparent. In contrast, Y79 cells pre-treated with retinoic acid/sodium butyrate, which attain a lesser degree of morphological differentiation, did not produce tumors over a 30 to 60 day-survival time (del Cerro et al., Brain Research, 12-22, 1992). We conclude that for PEDF and IPM, the degree of in vitro differentiation and the degree of mitotic arrest are independent features.

Original languageEnglish (US)
Pages (from-to)289-297
Number of pages9
JournalGrowth Factors
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1994

Fingerprint

Retinoblastoma
Pigments
Tumors
Butyric Acid
Tretinoin
Brain
Cells
Neoplasms
Transplantation
Survival
Research
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Seigel, Gail M. ; Tombran-Tink, Joyce ; Becerra, S. Patricia ; Chader, Gerald J. ; Diloreto, David A. ; Cerro, Constancia Del ; Lazar, Eliot S. ; Cerro, Manuel Del. / Differentiation of y79 retinoblastoma cells with pigment epithelial-derived factor and interphotoreceptor matrix wash : Effects on tumorigenicity. In: Growth Factors. 1994 ; Vol. 10, No. 4. pp. 289-297.
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Differentiation of y79 retinoblastoma cells with pigment epithelial-derived factor and interphotoreceptor matrix wash : Effects on tumorigenicity. / Seigel, Gail M.; Tombran-Tink, Joyce; Becerra, S. Patricia; Chader, Gerald J.; Diloreto, David A.; Cerro, Constancia Del; Lazar, Eliot S.; Cerro, Manuel Del.

In: Growth Factors, Vol. 10, No. 4, 01.01.1994, p. 289-297.

Research output: Contribution to journalArticle

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AU - Seigel, Gail M.

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AU - Chader, Gerald J.

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