Digital image analysis of lung transplant rejection in the rat: Quantitative differences between ischemic reperfusion injury in syngeneic grafts vs untreated allogeneic grafts

S. Levin, V. Lotano, B. Hausen, T. Wagner, D. Zeltsman, Mary Catherine Santos, L. Strande, E. Doolin, C. Hewitt

Research output: Contribution to journalArticle

Abstract

Objective: To investigate computerized digital image analysis (Mocha, Jandel) quantifying differences between ischemic/reperfusion injury in syngeneic grafts as opposed to alloimune graft rejection. Methods: Conventional bright field and novel high definition/SD microscopy with multiple oblique illumination (Edge, Inc.) was performed on various H&E and elastin stained specimens LEW to LEW syngeneic and UN to LEW (Rtl->Rtl') allogeneic single lung block grafts were performed. Nine images were captured and processed from each recipient (n=54). All recipients were examined at greater than two months post transplant. Results: Total alveolar air space in the syngeneic group was significantly (p<0.001) greater than the aliogeneic recipients (48.2 ±13.4% vs. 6.1±5.5%). Total interstitial tissue area was significantly less (pO.OOl) in the syngeneic group vs. the allogeneic group (51.9±13.4% vs. 93.9+ 5.5%). Analysis of arterioles demonstrated significant increases in vessel wall thickness when measured as a function of total vessel-diameter for the allografts compared to syngeneic grafts (0.288±0.028 vs. 0.140±0.021; p<0 002). The ratio of nuclear number to total tissue area as a measure of cellularity was significantly greater in the syngeneic group (0.009±0.001 vs. 0.006±0.003; p<0014) Conclusion: Significant differences were established for total alveolar air space, arteriolar wall thickness, and cellularity by computerized digital image.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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