Dihydropyridine-sensitive Ca2+ influx modulated by stretch in A7r5 vascular smooth muscle cells

Victor Ruiz-Velasco, M. Beatriz Mayer, Lin J. Hymel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We examined 45Ca2+ influx in A7r5 vascular smooth muscle cells under cyclical stretch and static conditions and compared the results obtained at resting membrane potential (2.5 mM [K+]o, Em = -58 mV according to uptake of [3H]tetraphenylphosphonium) with those under depolarizing conditions (70 mM [K+]o, Em = -27 mV). Application of 10% average strain (24% maximum) in cycles of 3 s on, 3 s off at resting Em caused a 5-fold increase in Ca2+ influx rate to a level similar to depolarized cells and depolarized, stretched cells. 1 μM (+)-isradipine blocked 90% of the stretch-or depolarization-activated Ca2+ uptake. When the cells were stretched under Na+-free conditions, a reduction, not activation, of Ca2+ influx rate occurred. Our results suggest that stretching of cultured aortic vascular smooth muscle cells enhances Ca2+ uptake through a voltage-dependent, dihydropyridine-sensitive Ca2+ entry pathway, whose activation by stretch is dependent upon extracellular Na+.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalEuropean Journal of Pharmacology
Volume296
Issue number3
DOIs
StatePublished - Feb 5 1996

All Science Journal Classification (ASJC) codes

  • Pharmacology

Fingerprint Dive into the research topics of 'Dihydropyridine-sensitive Ca<sup>2+</sup> influx modulated by stretch in A7r5 vascular smooth muscle cells'. Together they form a unique fingerprint.

  • Cite this