Dimensionless Parameters as Design Guidelines for Sterilization of Dead‐Ended Tubes

Jack H. Young, William C. Lasher

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although steam‐in‐place (SIP) is the primary process for sterilization of bioreactors, sterilization guidelines do not exist for equipment design and little quantitative data are available concerning critical parameters such as length, diameter, and orientation of deadlegs. This study correlated microbiological sterilization data with the critical dimensionless parameters governing buoyancy driven convective flow within dead‐ended tubes during SIP sterilization. These parameters were shown to be the overall Grashof number, Grov, and aspect ratio, Ar. Typical values of Grov for bioprocess equipment can range from 103 to 105, while Ar may vary from near 0 to 20 during sterilization of vertical tubes at 121 °C. Reproducible sterilization resulting in a 12 log reduction in B. stearothermophilus spores required an Ar of less than 7 when Grov was 0.7 × 105, whereas an Ar of 12 could be used when Grov was 2.8 × 105.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalBiotechnology progress
Volume11
Issue number3
DOIs
StatePublished - Jan 1 1995

Fingerprint

Guidelines
Equipment Design
Bioreactors
Spores
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Young, Jack H. ; Lasher, William C. / Dimensionless Parameters as Design Guidelines for Sterilization of Dead‐Ended Tubes. In: Biotechnology progress. 1995 ; Vol. 11, No. 3. pp. 312-317.
@article{a51aa21438ca4ebdb8e1ae23994fe99a,
title = "Dimensionless Parameters as Design Guidelines for Sterilization of Dead‐Ended Tubes",
abstract = "Although steam‐in‐place (SIP) is the primary process for sterilization of bioreactors, sterilization guidelines do not exist for equipment design and little quantitative data are available concerning critical parameters such as length, diameter, and orientation of deadlegs. This study correlated microbiological sterilization data with the critical dimensionless parameters governing buoyancy driven convective flow within dead‐ended tubes during SIP sterilization. These parameters were shown to be the overall Grashof number, Grov, and aspect ratio, Ar. Typical values of Grov for bioprocess equipment can range from 103 to 105, while Ar may vary from near 0 to 20 during sterilization of vertical tubes at 121 °C. Reproducible sterilization resulting in a 12 log reduction in B. stearothermophilus spores required an Ar of less than 7 when Grov was 0.7 × 105, whereas an Ar of 12 could be used when Grov was 2.8 × 105.",
author = "Young, {Jack H.} and Lasher, {William C.}",
year = "1995",
month = "1",
day = "1",
doi = "10.1021/bp00033a012",
language = "English (US)",
volume = "11",
pages = "312--317",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

Dimensionless Parameters as Design Guidelines for Sterilization of Dead‐Ended Tubes. / Young, Jack H.; Lasher, William C.

In: Biotechnology progress, Vol. 11, No. 3, 01.01.1995, p. 312-317.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dimensionless Parameters as Design Guidelines for Sterilization of Dead‐Ended Tubes

AU - Young, Jack H.

AU - Lasher, William C.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Although steam‐in‐place (SIP) is the primary process for sterilization of bioreactors, sterilization guidelines do not exist for equipment design and little quantitative data are available concerning critical parameters such as length, diameter, and orientation of deadlegs. This study correlated microbiological sterilization data with the critical dimensionless parameters governing buoyancy driven convective flow within dead‐ended tubes during SIP sterilization. These parameters were shown to be the overall Grashof number, Grov, and aspect ratio, Ar. Typical values of Grov for bioprocess equipment can range from 103 to 105, while Ar may vary from near 0 to 20 during sterilization of vertical tubes at 121 °C. Reproducible sterilization resulting in a 12 log reduction in B. stearothermophilus spores required an Ar of less than 7 when Grov was 0.7 × 105, whereas an Ar of 12 could be used when Grov was 2.8 × 105.

AB - Although steam‐in‐place (SIP) is the primary process for sterilization of bioreactors, sterilization guidelines do not exist for equipment design and little quantitative data are available concerning critical parameters such as length, diameter, and orientation of deadlegs. This study correlated microbiological sterilization data with the critical dimensionless parameters governing buoyancy driven convective flow within dead‐ended tubes during SIP sterilization. These parameters were shown to be the overall Grashof number, Grov, and aspect ratio, Ar. Typical values of Grov for bioprocess equipment can range from 103 to 105, while Ar may vary from near 0 to 20 during sterilization of vertical tubes at 121 °C. Reproducible sterilization resulting in a 12 log reduction in B. stearothermophilus spores required an Ar of less than 7 when Grov was 0.7 × 105, whereas an Ar of 12 could be used when Grov was 2.8 × 105.

UR - http://www.scopus.com/inward/record.url?scp=0028794752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028794752&partnerID=8YFLogxK

U2 - 10.1021/bp00033a012

DO - 10.1021/bp00033a012

M3 - Article

AN - SCOPUS:0028794752

VL - 11

SP - 312

EP - 317

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 3

ER -